To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果：用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒，实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
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