Methods Routine physiological and biochemical methods and real time PCR were used.
方法常规生理生化鉴定法和实时荧光pcr法。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.
探讨荧光mgb探针实时PCR技术检测经典型苯丙酮尿症的基因突变。
The facility provides technical services for the clients, which include Oligonucleotide Synthesis, Protein Sequencing, DNA Sequencing, Real Time PCR/Allele Detection.
该机构为客户提供寡核苷酸合成、蛋白质测序、DNA测序、实时PCR和等位 基因检测等技术服务。
In the paper, the rapid and accurate methods for detection and identification of food borne pathogens were established using real time PCR, RT-real time PCR and DNA chip.
本文运用实时荧光pcr、实时荧光rt - PCR和DNA芯片技术建立了快速检测食源性致病菌的方法。
Each lab now has a real-time polymerase chain reaction (PCR) machine, and one lab has two machines.
每个实验室都有一台实时多聚酶链式反应机，有一个实验室有两台这样的机器。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
The products of ruminant feed and cattle or sheep-derived feed from the enterprises and farms of 7 districts in Shaanxi province were detected by real-time PCR.
实时PCR对陕西省7个地市生产、经营和使用的反刍动物饲料产品和动物源性饲料产品（原料）进行了牛羊源成分检测调查。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Objective To quantitatively analyse the relationship between uncultivated phylotype AU126 and periodontal disease with real-time PCR.
目的运用实时P CR技术，对未获培养微生物au126与牙周病的关系进行定量分析。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
The molecule biological techniques DGGE, clone and real-time PCR were utilized to study prinimilarily the microorganism in 2 anaerobic ammonia oxidation reactors.
利用变性梯度凝胶电泳、克隆和实时PCR等分子生物学技术对2个厌氧氨氧化反应器中的微生物进行了初步研究。
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
用实时定量pcr检测标本线粒体dna的拷贝数。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time detection ensures accurate quantity, so FQ-PCR can be used to monitor the true state of HCMV infection and amplification.
FQ PCR可以准确定量检测HCMV的真实感染和复制情况，对于临床诊断与治疗有一定的指导意义。
Real-time PCR reaction; Amplification curve output; Ct data obtain;
进行实时定量PCR反应，建立标准曲线；
Objective: To investigate the effect of the high lipid, high bilirubin and hemolytic blood specimens on real time fluorimetry PCR of HBVDNA.
目的：探讨脂血、高胆红素和溶血标本对乙肝病毒DNA（HBVDNA）荧光定量测定结果的影响。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
This real-time PCR reaction system is highly specific, reliable, high sensitivity for detecting pathogenic fungus which could be useful for the diagnosis of clinical fungus infection.
建立的实时荧光PCR方法能特异、灵敏的检测临床病原真菌，为临床真菌感染的诊断提供了一种快速、准确的方法。
The autopsy samples, including heart, liver, spleen, lung, kidney and brain, were ground and detected by Real-time PCR. According to the standard curve, virus load of H5N1 AIV was calculated.
将人禽流感死亡病例的尸解标本，包括心、肝、脾、肺、肾、脑等组织标本研磨处理后，进行荧光定量pcr检测，并根据标准曲线推算H5N1禽流感病毒的病毒载量。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
Cytology on BKV infection was carried out and was compared with PCR. Cytology has less sensitivity and specificity than real-time quantitative PCR.
开展了BK病毒感染细胞学研究，并与PCR方法作了比较，认为细胞学检查灵敏度和特异度均低于定量pcr法。
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