Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
经pcr鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
The length and the results of restriction enzyme digestion indicate that the amplified products are respected.
各扩增产物长度和限制性酶酶切结果表明均为预期产物。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
Results:After being identified by PCR, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.
结果：经PCR，酶切及测序方法鉴定，该腺病毒-整合酶嵌合系统构建成功。
Methods: 20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR) technique.
方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（PCR）技术。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
CONCLUSION: Because of the speediness, simpleness and good specificity, the PCR combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of CMT1A.
结论：由于PCR -双酶切方法快速、简单、易操作，且特异性好，可作为CMT1A基因诊断的一种初筛方法。
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