Random hexamers prime reverse transcription at multiple points along with the transcript.
随机六聚体与转录物一起在多个点引发逆转录。
HBV replicates via a unique reverse transcription process.
HBV通过特有的逆转录反应进行复制。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
This dogma has since been shown to have exceptions, such as reverse transcription in retroviruses.
这种教条一直表现出的例外，如在反转录病毒。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
Similar results were obtained through detecting the expression of CXCR4 with reverse transcription-polymerase chain reaction.
反转录-聚合酶链反应法检测CXCR4的表达，亦得到相似结果。
Reverse transcription polymerase chain reaction technique for the detection of CTV in a single aphid or aphids has been developed.
检测橘蚜携带CTV的分子生物学反转录-聚合酶链式反应技术已建立，并已应用于检测橘蚜等蚜虫的单虫带毒情况。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
Influenza virus sample in cultured cells was treated for reverse transcription, amplification, fluorescent dye labelling, hybridization and scanning.
对待检样品进行随机逆转录和扩增，荧光标记，杂交，扫描分析。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
Methods The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR.
方法收集济南市儿童医院婴幼儿病毒性腹泻粪便标本，使用杯状病毒特异性引物对标本进行RT-PCR检测。
The gene expression levels for interstitial collagenase and tissue inhibitor of metallo-proteinase-1 (TIMP-1) were measured by reverse transcription polymerase chain reaction.
逆转录多聚酶链反应检测间质胶原酶及金属蛋白酶组织抑制因子- 1基因表达水平。
Methods differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.
方法采用差异显示反转录- P CR (DDRT - PCR)方法，寻找骨组织内与健骨冲剂疗效相关的有差异表达的基因。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
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