Methods differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.
方法采用差异显示反转录- P CR (DDRT - PCR)方法，寻找骨组织内与健骨冲剂疗效相关的有差异表达的基因。
Viral detection by reverse transcription polymerase chain reaction (RT-PCR) assay, and.
采用逆转录聚合酶链式反应(RT - PCR)检测病毒。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
A TC-RT-PCR method basing on reverse transcription with random primer facilitated the detection for those samples mixed infected by ASGV and ACLSV.
用随机引物反转录，通过TC-RT-PCR检测ACLSV和ASGV复合感染的梨样品也均获得了目标片段。
Methods HGV RNA in plasma of 84 IVDUs was detected by reverse transcription polymerase chain reaction (RT PCR).
方法采用逆转录聚合酶链反应(RT - PCR)检测84例静脉毒瘾者血浆标本。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay (RT-PCR).
方法采用逆转录—聚合酶链反应(RT -PCR)方法检测40例患者的肺癌组织ttf - 1基因的表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods The fecal specimens from children with acute nonbacterial gastroenteritis were collected and HuCV in the samples were detected by reverse transcription (RT)-PCR.
方法收集济南市儿童医院婴幼儿病毒性腹泻粪便标本，使用杯状病毒特异性引物对标本进行RT-PCR检测。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR).
用逆转录-聚合酶链反应（RT-PCR）法进行检测。
40 blind samples were examined by AGAR gel precipitation (AGP), hemagglutination inhibitory (hi) test and reverse transcription-polymerase chain reaction (RT-PCR).
对40份盲样采用琼脂扩散试验(agp)、血凝抑制试验(HI)和反转录聚合酶链反应(RT - PCR)进行了检测。
G1 gene sequence of m fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.
采用逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒基因组M片段G1区基因序列并测序。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
The mRNA was detected by using reverse transcription-polymerase chain reaction analyses(RT-PCR);
基因表达采用逆转录-聚合酶链式反应（RT-PCR）方法。
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