A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
SEC2 was separated and purified successively by ultrafiltration, salting out with ammonium sulfate, ion - exchange chromatography and molecular exclusion chromatography.
发酵液经超滤浓缩、硫酸铵沉淀、阴离子交换色谱和分子筛色谱，获得了纯度为90%的SEC2，总收率23%。
SEC2 was separated and purified successively by ultrafiltration, salting out with ammonium sulfate, ion - exchange chromatography and molecular exclusion chromatography.
发酵液经超滤浓缩、硫酸铵沉淀、阴离子交换色谱和分子筛色谱，获得了纯度为90%的SEC2，总收率23%。
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