CONCLUSION Sphingomyelin can be obtained from erythrocyte membrane by this method.
结论用此法可将红细胞膜中的鞘磷脂降解成神经酰胺。
OBJECTIVE To extract the sphingomyelin from erythrocyte membrane and hydrolyze it to ceramide.
目的从红细胞膜中提取鞘磷脂并降解成神经酰胺。
High sphingomyelin phosphodiesterase-producing Actinomucor elegans mutant(AE255-6) was obtained by radiation of CO2 laser.
用CO2激光辐射筛选培育出高产鞘磷脂酶的工程菌——雅致放射毛霉变异株AE255-6。
METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood, erythrocytolysis, dehydration, dry, et al, and then hydrolyzed to ceramide by phospholipase C.
方法分离猪血细胞，用溶血，脱水，干燥等方法提取鞘磷脂，并用磷脂酶C降解鞘磷脂成神经酰胺。
METHOD Sphingomyelin was prepared through the process of separating erythrocyte from blood, erythrocytolysis, dehydration, dry, et al, and then hydrolyzed to ceramide by phospholipase C.
方法分离猪血细胞，用溶血，脱水，干燥等方法提取鞘磷脂，并用磷脂酶C降解鞘磷脂成神经酰胺。
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