The tissue culture method can provide a basis for haploid breeding.
本发明方法可为单倍体育种提供基础。
The optimum tissue culture method is tube miniature ginger propagation.
适宜的组培快繁方式为试管微型姜快繁法。
Methods The hypertrophic scar fibroblasts were cultured with tissue culture method.
方法用组织块法培养人瘢痕成纤维细胞。
The invention discloses a tissue culture method and a special culture medium of a Spanish dagger anther.
本发明公开了一种凤尾兰花药组织培养的方法及其专用培养基。
Results the breast carcinoma cells could be cultured by these two methods with a success rate of 90% in the tissue culture method and that of 20% in the cell culture method.
结果两种方法均能得到乳腺癌原代细胞，组织块培养法的成功率为90.0%，细胞培养法的成功率为20.0%。
Methods: Using pathological section dyeing with PAS and alive tissue culture method to examine fungus, and next analyzing the characteristics in clinical and endoscope for the positive case.
方法：采用病理切片PAS染色和活组织培养法检测真菌，对阳性病例分析临床及内镜特点。
In adopting tissue culture method, the influence of different explant types, hormone constitutions, sugar concentrations on pepper axillary buds formation and mediums on bud rooting were studied.
采用组织培养方法，研究了不同外植体类型、激素组合、蔗糖浓度对辣椒腋芽生成以及不同培养基对腋芽生根的影响。
To tap their toughness, or finding method in tissue culture more flexible corporate culture, try these four tips.
要挖掘自己的韧性，或寻找方法在组织内培养更具韧性的企业文化，试试下面这四个秘诀。
At various stages in the process, the number of cells are increased by a laboratory method called a "tissue culture".
在这个过程的不同阶段，称之为“组织培养”的实验室方法导致细胞数量增加。
METHODS: Cell culture technique and tissue engineering method were used.
方法：采用细胞培养技术和组织工程学方法。
The method of tissue culture is used to induce the cotyledons of cucumis melon to become callus and then adventitious buds.
用组织培养的方法使甜瓜子叶脱分化形成愈伤组织，继而在分化培养基上形成不定芽。
Methods Human fetal limbal epithelial tissue was cultured in vitro by enzymatic digestion culture method and the morphological characteristics of cultured cells were recorded.
方法应用消化培养法对人胎儿角膜缘上皮组织进行体外培养并观察记录培养细胞的生长特性；
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
Method: High efficiency liquid phase chromatography and plant tissue culture were applied.
方法：采用高效液相色谱法和植物组织培养的方法。
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
组织块培养法对于原代细胞的培养要优于酶消化法。
Besides, a method of carcinoma tissue asepsis before culture was investigated, a best treatment schedule was worked out, namely, a model of 75% alcohol-metronidazolevarious antibiotics.
此外，为保证结直肠癌组织培养不污染，本工作对癌组织培养前的无菌消毒方法做了探索，找出最佳处理方案，即“75％酒精-灭滴灵-多种抗菌素”模式。
Subculture is an important link in the process of the tissue culture and the long-term subculture is the essential method for conservation in vitro of germplasm.
在植物组织培养过程中，继代培养是其中的重要环节，而长期继代则是种质资源离体保存的必要手段。
The successful rate of the enzymatic digestion culture method was 83.3%, which was prior to the tissue pieces culture method significantly.
经酶消化法短期培养成功率为83.3%，明显高于组织块培养法。
CONCLUSION: The fibroblasts bank of mini fragrant pigs construct by using cell culture technique and tissue engineering method.
结论：作者通过细胞培养和组织工程学的方法，建立了小型香猪角质形成细胞库。
Objective To study the method of tubular scaffold and the application of dynamic implantation and culture in vascular tissue engineering.
目的探索体外构建组织工程血管及细胞种植的方法。
Method:Primary cells of human degenerative AF were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法，体外单层培养人退变纤维环原代细胞。
The method using in vitro irradiation seems to be more effective than conventional irradiation, tissue culture or backcross for improving a few characters in a variety.
因此，对于品种个别性状的改造，离体辐照可能比其它方法更有效。
Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.
方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。
The invention discloses a Chinese yew-seedling tissue culture medium and the breeding method, made up of nutritious agent, growth promoter, stabilizing agent and microelement.
本发明公开了一种红豆杉种苗组织培养基及其种苗繁育方法，它由营养剂、生长促进剂、稳定剂、微量元素配制而成。
Method: The method of cell and tissue culture was utilized to carry out an observation on the cytopathy effect(CPE) led by influenza virus FM1 with the aerosol of different concentrations in vitro.
方法：体外试验采用组织细胞培养法，观察不同浓度药物对流感病毒FM1病毒株致细胞病变（CPE）的作用；
The techniques, developed in 1970, for plant cell and tissue culture provide the selection of resistant varieties with an advanced method.
植物组织或细胞培养结合理化因素的处理，可大大提高变异的范围和频率。
Method : The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.
方法 ：将摩西球囊霉接种于半夏无菌组培苗，于不同时间取样，通过染色镜检法观察丛枝菌根形成过程。
Method : The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.
方法 ：将摩西球囊霉接种于半夏无菌组培苗，于不同时间取样，通过染色镜检法观察丛枝菌根形成过程。
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