Objective To establish and optimize the two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease(ESRD).
目的建立和优化终末期肾病患者血清蛋白质组研究的双向电泳及相关技术，并与正常血清蛋白图谱比较。
To explore the methods of sample preparation of two-dimensional electrophoresis of astrocyte cultured in vitro, for improving its resolution and reproducibility.
【目的】探索体外培养星形胶质细胞双向电泳样品制备方法，提高电泳分辨率和重复性。
Objective: To separate human kidney phosphoproteome by two-dimensional gel electrophoresis.
目的：利用二维凝胶电泳法分离人肾组织磷酸化蛋白质组。
Methods nuclear matrix proteins were extracted under high salt extraction procedures, and changes of nuclear matrix proteins were analysed by SDS PAGE and two dimensional (2d) electrophoresis.
方法应用高盐提取法抽提细胞核基质蛋白，SDSPAGE、双相电泳(2d)法分析白血病细胞与正常骨髓细胞核基质蛋白的变化。
Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳（2DPAGE）与质谱（MS）是分辨和鉴定蛋白的最常用的方法，但也存在有缺点。
Two-dimensional electrophoresis was used to analyze differential proteins between the perfect and imperfect flowers in Japanese apricot(Prunus mume Sieb. et Zucc).
应用双向电泳技术对果梅完全花与不完全花的蛋白质组分进行了比较分析。
Methods: The contents of serum HDL subclasses in 83 CHD subjects were determined by two dimensional gel electrophoresis associated with immunodetection method.
方法：采用双向电泳免疫印迹检测法分析了83例CHD患者血清hdl亚类组成及相对百分含量。
Methods The soluble antigens of the parasites were analysed by means of SDS PAGE and two dimensional gel electrophoresis.
方法用SDS-PAGE和双向电泳方法，对日本血吸虫两性成虫全虫可溶性抗原进行分析。
And proteomics technology based on two-dimensional electrophoresis is an effective approach to discover the changes in the states of protein phosphorylation.
以二维电泳为基础的蛋白质组学是发现蛋白磷酸化状态改变的有效途径。
Based on the method of o Farrell, an improved procedure for the two dimensional gel electrophoresis of leaf proteins is presented.
通过对传统的双向电泳方法进行改进与优化，得到了一种适合于分析植物叶片蛋白质的双向电泳新方法。
Proteomic techniques mainly include two-dimensional electrophoresis, mass spectrometry and bioinformatics.
蛋白质组研究技术主要包括双向电泳、质谱和生物信息学。
Methods Using high resolution two dimensional polyacrylamide gel electrophoresis, the nuclear matrix proteins of 3 normal livers and 8 HCC were compared.
方法用双向电泳方法比较了3例正常肝和8例HCC核基质蛋白成分。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
Proteomics has its origins in two dimensional gel electrophoresis (2 DE), a technique developed more than twenty years ago.
蛋白质组学部分承用了创立于二十多年前的二维电泳技术。
Two-dimensional gel electrophoresis(2DE) is a key technology for proteomics, however, it often ended in defect gels in real experiments.
双向凝胶电泳是蛋白质组学研究中的关键技术之一，涉及较多的实验步骤和试剂，常出现缺陷胶而导致实验失败。
The change of proteome of rat liver is analyzed by two-dimensional gel electrophoresis (2-DE).
通过双向凝胶电泳技术分析大鼠的肝脏蛋白质组变化。
Objective:To establish and optimize two-dimensional electrophoresis (2-DE) for proteonomic analysis of the human lens and to promote resolution and reproducibility.
目的：初步建立人晶状体蛋白质组研究中的双向电泳分离技术，提高晶状体蛋白分辨率和重复性。
In addition, by means of two-dimensional electrophoresis, mass spectrometry and other proteomic methods to construct zebrafish embryo protein expression profile in different periods.
借助双向电泳、质谱分析等蛋白质组学分析方法，构建斑马鱼胚胎的蛋白质表达谱。
To develop and optimize a two-dimensional(2-DE) gel electrophoresis condition for Candida albicans proteome analysis.
建立并优化白念珠菌总蛋白质组分析的二维电泳条件。
The polypeptide patterns of the eggs of Anopheles sinensis and An. anthropophagus were compared with two-dimensional electrophoresis in polyacrylamide gel.
用二维电泳法比较分析两种按蚊卵的蛋白质多肽图谱。
To establish analysis methods of two-dimensional (2-DE)gel electrophoresis for human osteosarcoma.
目的： 建立人骨肉瘤蛋白质组双向电泳图像分析方法。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
目的探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
To establish a set of two-dimensional polyacrylamide gel electrophoresis (2-DE) techniques for proteomic research of hepatocellular carcinoma (HCC) tissues.
优化双向凝胶电泳的实验步骤，总结出一套适用于原发性肝癌组织的双向凝胶电泳技术。
OBJECTIVE To study the effects of warm and tonify kidney-yang herbs on the liver mitochondria proteome of the kidney-yang deficiency rats by two-dimensional difference gel electrophoresis (2-D DIGE) .
目的：应用凝胶内差异显示电泳技术和质谱技术研究温补肾阳药对肾上腺切除的肾阳虚大鼠肝线粒体蛋白质组的影响。
OBJECTIVE To study the effects of warm and tonify kidney-yang herbs on the liver mitochondria proteome of the kidney-yang deficiency rats by two-dimensional difference gel electrophoresis (2-D DIGE) .
目的：应用凝胶内差异显示电泳技术和质谱技术研究温补肾阳药对肾上腺切除的肾阳虚大鼠肝线粒体蛋白质组的影响。
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