Using the organ of nonthorny big fruit seabuckthom as the experimental material, this paper studied the technology of the tissue culture of nonthorny big fruit seabuckthom.

本文以无刺大果沙棘—太阳品种的幼嫩器官为组织培养的繁殖材料，研究了无刺大果沙棘的组织培养技术。

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Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.

方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。

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Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.

方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。

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