They then checked 229 positive patients with the gold-standard laboratory test, a Western blot. More than 10% of the rapid tests were giving false bad news.
之后，他们又应用金标准试验——蛋白印迹法(Western blot)检测了229名阳性患者，结果表明快速检测结果中有超过10%为假阳性。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
Maximal binding volume of glucocorticoid receptor (GR) in hepatic tissue was assayed by radio-ligand binding assay and protein content was assayed by Western blot.
肝组织GR采用放射性配体结合分析法测定其最大结合容量以及用免疫印迹法测定其蛋白含量。
The aliquot of proteins was subjected to SDS-PAGE, followed by Western blot analysis in which individual sera were tested for primary antibody.
用肺癌患者及健康对照者的血清作第一抗体进行蛋白印迹检测。
Cell lysates were collected for Western blot analysis.
收集到的细胞溶解液均进行免疫印迹分析。
To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot, the protein marker and purified recombination protein were used to analyzed.
采用蛋白质分子量标准物和纯化的重组蛋白质进行半干式蛋白质印迹，探讨半干式电泳印迹的多种因素对印迹效果的影响。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株，ELISA方法测定重组蛋白表达水平，集落形成实验测定重组蛋白活性。
Its immunoreactivity was analyzed by Western blot, ELISA and blocking ELISA using MAb and positive serum.
采用单克隆抗体和阳性血清经elisa、阻断elisa、免疫印迹分析重组蛋白的免疫反应性。
Western blot analysis of the GST-TM fusion protein using sera from subjects with crustacean allergy confirmed that TM is the major allergen of Chinese mitten crab.
通过与甲壳类过敏患者血清的免疫印迹反应，证实融合表达的原肌球蛋白具有过敏原性，表明原肌球蛋白是蟹类的主要过敏原之一。
Western blot showed the good immunological specificity of the IgY.
经免疫印迹分析，具有良好的免疫学特异性。
Methods To analyze the clinical data of 15 AIDS patients identified by western blot test.
方法分析经免疫印迹检测证实的15例aids病人的临床资料。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
The content of heat shock protein 70 in myocardium was detected with western blot method.
采用蛋白印迹法测定心肌细胞中热休克蛋白70含量。
The expression level of NGAL in 5 esophageal carcinoma cell lines was also examined by immunofluorescence and western blot.
运用免疫荧光和蛋白印迹技术检测了5种食管癌细胞系中NGAL的表达。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
结果表明：所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合，但在免疫印迹分析中仅与阳性血清发生特异性结合。
Objective To develope a western blot of human plasma factor V (FV) and conduct immunoblot analyses on riasma FV from a family with hereditary FV deficiency.
目的建立人血浆因子V（FV）蛋白免疫印迹分析，对遗传性FV缺乏症家系成员血浆FV含量进行分析。
The expression of the above three HA DNA vaccines was verified in 293t cells by Western blot analysis.
用这3种重组质粒分别转染293t细胞，蛋白表达经蛋白质印迹检测得到确认。
UAF were analyzed with Western Blot, integrated density value were determined and relative excretion rate was counted.
采用免疫印迹法测定uaf，并测定其积分密度值，计算相对排泄率。
Results The RSV G glycoprotein was expressed by the recombinant vaccinia virus, which was determined by Western blot with anti-G protein specific monoclonal antibody.
结果RSV地方株G蛋白在痘苗病毒中获得较好的表达，表达产物的糖基化程度较高，且该重组病毒免疫家兔可诱发特异性抗体产生。
Methods HIV antibody were screened by ELISA and confirmed by western blot( WB)in 2 272 patients diagnosed with tuberculosis.
方法对2 272例诊断为结核病的住院病人进行HIV抗体检测，初筛阳性者进一步做免疫印迹试验（WB）。
Methods: the 233 drug users were surveyed using the same questionnaire, HIV antibodies were screened and confirmed using enzyme-linked immunosorbent assay (ELISA) and Western Blot (WB) respectively.
方法：采用同一问卷对强制戒毒所233名吸毒者进行调查，并用酶联免疫吸附试验(ELISA)和确认试验(WB)对吸毒者进行HIV初筛和确认。
Note the use of strategies to help students will learn the chemical knowledge into the written record, leaving the thinking of the Western blot, for learning and memory and understanding;
运用笔记策略，有助于学生将所学的化学知识转化为书面记录，留下思考的印迹，促进学习的记忆和理解；
Methods MMP-9 and TIMP proteins were examined by using zymographic analysis and Western blot in 41 cases with ESCC.
方法免疫组化法分别检测41例食管鳞癌患者的癌及相应正常组织中MMP-9和TIMP的表达变化。
The fusion protein has been proved having immunogenicity when detected by Western blot, which demonstrates that it may be useful in the development of porcine pleuropneumonia subunit vaccine.
该融合蛋白的成功表达为猪传染性胸膜肺炎亚单位疫苗的研制奠定了基础。
Common technologies used for HCP analysis include ELISA (Enzyme-linked Immunosorbent Assay), one-dimensional or two-dimensional gel electrophoresis, Western Blot and Mass Spectrometry.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验)，一维或二维凝胶电泳，免疫印迹和质谱分析。
The misuse and re-use of western blot bands violated the editorial policy of Journal of Cell Science, and so we must retract this article.
作者表示，该论文滥用和重复使用他们发表的其他论文中的蛋白 免疫印迹图片，违背了《细胞科学期刊》的编辑政策，必须撤销。
Method:The protein of p16 was examined by Western blot in 6 cases of normal pituitary and 40 pituitary adenomas.
方法应用蛋白质杂交技术分析P16在垂体腺癌中的表达。
Western Blot assay shows the purified antibody have higher specificity.
实验表明纯化后的抗体具有较高特异性。
Western Blot assay shows the purified antibody have higher specificity.
实验表明纯化后的抗体具有较高特异性。
应用推荐