Then it was induced to expression and identified by SDS-PAGE and Western-blotting, and it was purified.
然后诱导表达，用SDS - PAGE及蛋白印迹进行鉴定，然后进行蛋白纯化。
The Western-blotting analysis showed the expressed protein could react with sera from tuberculosis patients specifitally.
免疫印迹分析证实目的蛋白与阳性结核病患者抗血清产生特异性反应。
The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.
骨形态发生蛋白2与骨桥蛋白检测：采用免疫组织化学染色与蛋白印迹方法。
Western blotting --- A technique analogous to Southern blotting, used for detection of proteins, usually by immunological methods.
蛋白质印迹法：蛋白质分子从电泳凝胶转移到固相介质，然后用抗体进行免疫检测的技术。
Use as a blocking reagent to evaluate the specificity of antibody reactivity in Western blotting and immunohistochemistry protocols.
在免疫印迹和免疫组化实验中，使用一个封闭试剂可以评估这个抗体反应的特异性。
The results of Western blotting showed that protein expression levels of MYOG and MYOD also changed as same as the gene expression levels.
免疫印迹杂交结果显示MYOG和MYOD的蛋白质表达水平也发生了与基因表达相一致的变化。
The presence of protein was measured by western blotting.
蛋白的测定采用蛋白质印迹法。
The new antibody conjugates are designed for Western blotting applications, which use gel electrophoresis to separate proteins from a sample of cell or tissue based on molecular weight and size.
新的抗体连接方法是为蛋白质印迹法专门设计的。后者是一种凝胶电泳法可根据细胞和组织样本的分子重量对其进行分离。
Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis.
结果：经荧光显微镜、流式细胞仪及免疫印迹法检测，证实AIRE基因得到了稳定表达。
Methods Immunohistochemical technique, image analysis, radioimmunoassay and Western blotting were used.
方法采用免疫组织化学技术、图像分析、放射免疫和免疫印迹法。
Western blotting also revealed an increase in HO-1 protein levels(P<0.01) but no change in HSP70 protein expression.
蛋白印迹也显示HO-1蛋白水平增加（P<0.01），但HSP70蛋白表达无明显变化。
Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.
目的：确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
Western blotting was used to detect protein expression of VEGF.
用免疫蛋白印迹法检测细胞VEGF蛋白表达。
The Western blotting analysis showed the expressed protein could react with the serum from tuberculosis patients speicfically.
免疫印迹分析证实目的蛋白与阳性结核病患者抗血清产生特异性反应。
We then detected EZH2 protein expression in the esophageal squamous carcinoma, paracarcinomatous and normal tissues by Western blotting.
方法 采用免疫组织化学染色法、免疫印迹法，分析检测食管癌标本中EZH2蛋白的表达。
In vitro the interaction between BNP23 and PBP1 was ascertained by means of the protein-protein interaction and western blotting.
经体外蛋白质—蛋白质之间的相互作用和蛋白质印迹杂交方法进一步验证了PBP1和BNP23的相互作用；
Immunocytochemistry and western blotting technology were used to identify the cell phenotype.
用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
Methods Specific immunologic affinic cell and Western blotting technology were performed to detect the specific neurologic antibody.
目的探讨检测特异性神经元抗体对诊断副肿瘤性神经系统疾病意义。
The viral NP and F proteins revealed the same patterns by Western Blotting with specific monoclonal antibodies.
二者的结构多肽NP蛋白和F蛋白用特异性单克隆抗体免疫转印技术分析，未显示差异。
Western blotting and immunohistochemical were applied to exploring the expression of the cell-cycle regulating factors during the free nuclei mitosis failure.
并进一步利用免疫印迹、免疫组化技术探讨了细胞周期相关调控因子在游离核分裂关键时期的表达。
As detected by Western blotting, TRPV1 expressions in the brain and skeletal muscle of the transgenic mice were significantly higher than the wild-type littermates.
经蛋白免疫印记法证实，与同窝野生型小鼠相比，转基因小鼠的脑组织和肌肉组织中TRPV1的表达显著升高。
The purified protein could specificially react with FMDV infection antibodies in Western blotting assay, but no reaction with the immune antibodies induced with vaccine.
能与口蹄疫病毒感染血清发生特异性反应，而不能与疫苗免疫动物血清发生反应；
Study protein-protein interaction by native and denaturing gel electrophoresis, western blotting, and immunology precipitation.
研究蛋白质-蛋白质相互作用，由本地和变性凝胶电泳免疫印迹，免疫沉淀。
Results: After transfection of the antisense ERCC1, Northern and Western blotting indicated that the RNA and protein expression level of ERCC1 was obviously decreased.
结果：卵巢癌细胞转染反义ERCC1基因后，ERCC1基因转录水平、蛋白表达水平明显下降。
This product has been optimized for use as a secondary antibody in Western blotting applications.
该抗体已经过优化，可以作为二抗用于western blot实验。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
Protein tyrosine phosphorylation in platelets was assayed by Western blotting and platelet aggregation was assayed by nephel omete r.
用蛋白质免疫印迹法 （Westernblotting）测定血小板内蛋白酪氨酸磷酸化的表达。
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