本文主要介绍时间分辨荧光免疫技术基本原理、基础试剂、基本技术以及近年来临床应用。
This article reviewed some aspects about it including fundamental principle, basic reagent, basic technique and the its clinical application in recent years.
这就经常意味着增菌培养试验需要优先于免疫荧光显微技术。
This usually meant that enrichment culture techniques were required prior to immunofluorescence microscopy.
方法采用细胞免疫荧光技术通过免疫荧光显微镜和激光共聚焦显微镜观察HPK1是否在大鼠脑细胞中存在；
Methods Immunofluorescence technique was used to investigate whether there was HPK1 in hippocampus neuron under the fluorescence microscope and the confocal microscopy.
本文以藻红蛋白为荧光探针，探讨了藻红蛋白荧光标记技术及荧光探针在烟草花叶病毒免疫检测中的应用效果。
The fluorescence labeling technique of phycoerythrin and application for the immunoassay of Tobacco mosaic Virus were studied in this research using it as a labeling probe.
并采用免疫荧光抗体染色技术对5例dmd,2例BMD肌细胞膜上抗肌营养不良蛋白的表达观察分析，以2例正常人的肌组织作为对照。
The patients from 5 DMD and 2 BMD were detected by immunofluorescence technique for analyzing dystrophin located in muscle cell membrane, compared with 2 normal males.
目的：观察超声成像及间接免疫荧光技术在骨髓基质细胞移植治疗缺血心肌病方面的评估价值。
AIM To observe the evaluation value of ultrasonic imaging and indirect immunofluorescence technique in the treatment of ischemic cardiomyopathy with marrow stroma cells MSCs transplantation.
现代荧光免疫分析技术主要包括荧光偏振免疫分析(FPIA)和时间分辩荧光免疫分析(TRFIA)两种技术。
Modern fluorescence immunoassay, which includes fluorescence polarization immunoassay (FPIA) and time-resolved fluorescence immunoassay (TRFIA), also plays an important role in these subjects.
介绍了应用激光扫描共聚焦显微镜，结合免疫荧光技术研究卵母细胞和早期胚胎细胞骨架的方法。
This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique.
本文目的是探讨耳蜗组织免疫荧光染色及激光共聚焦显微镜技术的应用。
The purpose of this article is to explore The Organization of cochlear immunofluorescent staining and laser scanning confocal microscope technology applications.
荧光共振能量转移(FRET)技术作为一种高效的光学“分子尺”，在生物大分子相互作用、免疫分析、核酸检测等方面有广泛的应用。
Fluorescence resonance energy transfer (FRET) has broad applications in the study of the interactions of biological macromolecules, immunoassay, nucleic acid detection and so on.
方法：采用细胞免疫荧光染色技术和流式细胞术。
Methods Flow cytometry and immunofluorescence staining technique were used.
方法应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。
Methods Cell culture, immunohistochemistry, nucleus fluorescence labeling, fluorescence retrograde labeling and enzymatic histochemistry technique were used.
探讨以计算机图像处理技术将普通荧光显微镜采集的同视野单标记免疫荧光图像合成为双标记图像的方法。
The present study investigated the methodology of synthesizing two single-staining immunofluorescence images into one overlapped double-stained image by using computerized image-processing techniques.
方法免疫组织化学和免疫荧光双标记技术。
Methods Immunohistochemical and double immunofluorescence techniques were used in the study.
结论视网膜血管消化铺片联合免疫荧光组织化学技术是可行的，为视网膜血管性病变的研究提供了重要的途径。
Conclusion retinal digest preparations combined with immunofluorescence. was feasible and provided an important method for the studies of retinal vascular diseases.
方法：采用受体放射配基结合分析、受体-DNA结合分析及免疫荧光技术。
Methods: receptor radioligand binding assay, receptor DNA binding assay and immunofluorescence methods were used.
方法：免疫荧光组织化学双重标记技术。
METHODS: Immunofluorescence histochemical double-staining method was used.
运用免疫荧光和蛋白印迹技术检测了5种食管癌细胞系中NGAL的表达。
The expression level of NGAL in 5 esophageal carcinoma cell lines was also examined by immunofluorescence and western blot.
方法用流式细胞仪检测缺血缺氧后不同时间点星形胶质细胞细胞周期变化，并用荧光免疫细胞化学技术测定胶质细胞纤维酸性蛋白（GFAP）和增殖细胞核抗原（PCNA ）的表达水平。
Methods We measured the astrocyte cell cycles in different time after ischemia and anoxia by flow cytometry and detected the levels of GFAP and PCNA with fluorescence immunocytochemistry.
尽管荧光性抗体试验或免疫组化技术也为孢子丝菌病提供了快速的诊断方法，但目前它们还不能被大多数临床实验室所采用。
Although the fluorescent antibody or immunohistochemical techniques also provide a rapid diagnosis of sporotrichosis, they are not available in most clinical laboratories.
用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
本文对这一技术的研究进展作了评述，介绍了荧光偏振免疫分析技术的基本原理、优越性、示踪物结构设计、制备、及其应用。
The review described the development of this technique. The principle and superiority of FPIA, tracer's designed and prepared, and its applications were introduced.
目的：利用时间分辨荧光免疫分析(TRFIA)技术，建立人血清ck - MB的快速定量检测方法。
AIM: To make a fast quantitative detection of Creatine Kinase-MB Isoenzyme (CK-MB) based on time-resolved fluoroimmunoassay (TRFIA).
方法：荧光金(FG)逆行追踪结合5 HT免疫荧光组织化学染色的双标技术。
METHODS: Fluoro Gold (FG) retrograde tracing combined with serotonin (5 HT) immunofluorescence histochemical staining method was used.
应用免疫金银染色技术（IGSS）和间接荧光抗体试验（IFAT）对日本血吸虫、华支睾吸虫和卫氏肺吸虫进行抗原定位的研究。
The antigen location of Schistosoma japonicum, Clonorchis sinensis and Paragonimus westermani were studied by using immunogold-silver staining(IGSS) and indirect fluorescent antibody test(IFAT).
方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
用时间分辨荧光免疫分析技术（TRFIA）对乙肝五项定量分析；
Time-resolved fluoroimmune assay(TRFIA) was used to measure the quantitation of HBV markers in serum.
方法应用免疫荧光组织化学双标记染色技术，在激光共聚焦扫描显微镜下观察染色结果。
Methods Double label immunofluorescence histochemical staining and confocal laser_scanning microscope were used in the experiment.
因此，我们尝试应用新的血清学技术，如荧光偏振免疫检测，来识别个别已受感染但对传统检测不起反应的牲口。
We therefore sought to apply novel serological techniques, such as fluorescence polarisation assay could identify infected individuals that were non-reactors in the conventional tests.
方法：采用逆转录聚合酶链反应(RT - PCR)、流式细胞仪和免疫荧光细胞染色分析技术分析肺上皮细胞系a549细胞PAR s的表达情况。
METHODS: We used RT-PCR, flow cytometry and immunofluorescence cell staining techniques to observe the expression of PARs on A549 cells.
应用推荐