Objective: To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.
目的：建立蟾皮中蟾蜍噻咛的含量测定方法，比较不同产地及养殖方式对蟾皮中蟾蜍噻咛含量的影响。
Results: Time and concentration of poison dying all had effect on the myocardial contracts dint and myocardial enzymogram of Bufo bufo gargarizans.
结果：除草剂丁草胺的染毒时间和浓度均可影响蟾蜍心肌收缩力和心肌梅谱。
To study the effects of UVB on development of early stage embryos of amphibian, two-cell stage embryos of Bufo bufo gargarizans Cantor were irradiated by UVB with various intensity and time.
为了研究UVB对两栖类动物早期胚胎发育的影响，以中华大蟾蜍为研究对象，用UVB分别以不同强度和不同时间照射其二细胞期胚胎，观察记录胚胎的发育过程、统计死亡率并在形态学上对UVB照射诱导的畸胚进行分析。
Conclusion: Herbicide butachlor can change myocardial physiological activity of Bufo bufo gargarizans, suggesting that herbicide should be used with great attention to environmental protection.
结论：除草剂丁草胺能改变蟾蜍心肌生理活动，提示使用除草剂应注意环境保护。
Conclusion: Herbicide butachlor can change myocardial physiological activity of Bufo bufo gargarizans, suggesting that herbicide should be used with great attention to environmental protection.
结论：除草剂丁草胺能改变蟾蜍心肌生理活动，提示使用除草剂应注意环境保护。
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