Objective To explore the possibility of achieving the highest resolution by using combination of anion and cation exchange chromatography.
目的:探索以阴、阳离子交换色谱串接组合为方式,进行高分辨率分离纯化蛋白质的可行性。
PurposeTo explore the possibility of combination of anion and cation exchange chromatography by using the polarity organic solvents in eluent.
目的研究极性有机溶剂辅助阴、阳离子交换色谱串接组合纯化蛋白质的可行性。
The expression supernatant was purified with cation exchange chromatography to single peak and SDS-PAGE indicated that the relative molecular mass was 6 500.
表达上清经阳离子交换层析分离纯化为单一组份,SDS - PAGE显示相对分子质量为6 500。
The iron-binding capacity of lactoferrin was 87.7% produced by ultrafiltration and strong cation exchange chromatography. Its iron saturation point was 15.1%.
采用超滤-离子交换色谱法制备的乳铁蛋白的铁结合能力为87.7%,铁饱和度为15.1%。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
In addition, an original heterogeneous polymerization method, based on displacement chromatography, has been tested. We named it "polymerization reaction in cation exchange chromatography".
此外,建立了一种原创的基于置换色谱的非均相聚合方法——阳离子交换色谱柱上聚合反应。
Based on this media, a new hydrophilic weak cation exchange stationary phase(WCX) for high(performance) liquid chromatography was synthesized by a new chemical modification method.
该微球经化学改性后得到一种亲水性良好的新型高效弱阳离子交换色谱固定相。
Cation - anion exchange resin can crack and disassemble bond - chromatography, doff the bond - large - molecule and dissociate paclitaxel.
阴阳混合离子交换树脂可以使键合态紫杉醇发生裂解,脱去键合的大分子物质,游离出紫杉醇。
The refolded endostatin was then purified by cation-exchange chromatography.
复性结束后用阳离子交换层析纯化。
The refolded endostatin was then purified by cation-exchange chromatography.
复性结束后用阳离子交换层析纯化。
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