BAC clones corresponding to telomeres, as well as to the centromere position and the gap sizes between contigs, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH).
端粒克隆、着丝粒克隆以及重叠群之间空缺的大小都经过粗线期染色体荧光原位杂交鉴定。
Transpositional points between chromosome 6 and 12 were anchored in 1 and 3 BAC clone's pool respectively by fluorescence in situ hybridization technology of pachytene chromosomes.
通过粗线期染色体荧光原位杂交技术,将发生易位的第6号和第12号染色体的易位点分别锚定在1个和3个BAC克隆库中。
Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method.
目的探讨用多色荧光原位杂交(M FISH)技术检测的易位和双着丝粒染色体畸变的差异。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
Methods:Multicolor fluorescence in situ hybridization (M-FISH) was applied to check the chromosome status in oocytes for chromome 13,16,18,21 and 22.
方法:取试管婴儿助孕技术后未能受精成功的卵细胞,采用多色荧光原位杂交方法检测卵细胞13,16 ,18,2 1和2 2号染色体的情况。
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