Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应（PCR），这是一项扩增特定DNA序列的重要技术。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
方法：采用聚合酶链反应(PCR)技术，检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBVDNA阳性的上海籍HBV感染者的基因型。
Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA测序来加以鉴定。
PCR-chip is a function biochip for DNA polymerase chain reaction. The temperature control section is so important that it decides the performance, dimension, integration of the whole chip.
P CR芯片是进行DNA聚合酶链式反应的芯片，温控部分的搭构是决定整个PCR芯片性能、尺寸、集成化的重要组成部分。
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restriction fragment-length polymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.
酚-氯仿法从外周血中提取基因组dna，聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法检测CYP11 B2基因C - 344t多态性。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods DNA of CT and UU was detected with polymerase chain reaction(PCR) technique.
方法采用聚合酶链反应技术（PCR）检测沙眼衣原体和解脲支原体的DNA。
Objective To establish DNA typing for HLA-DR antigens by polymerase chain reaction with sequence-specific primers (PCR-SSP).
目的采用顺序特异引物聚合酶链反应（PCR -SSP）建立人类白细胞抗原DR位点的DNA分型方法。
Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.
目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。
Methods Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR).
方法使用菌落原位杂交、DNA打点杂交和PCR扩增方法。
HLA-DR2, DR7, DR9 genotyping by polymerase chain reaction with sequence-specific primers (PCR-SSP)was carried out for 35 individuals and 4 cell lines DNA.
采用顺序特异性引物聚合酶链式反应（PCR-SSP）DNA分型技术，首次对35例肾移植供受者和4份标准DNA进行HLA-DR2、DR7、DR9基因配型。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ - PCR)的检测精度超过常规的PCR。
Objective:To evaluate the clinical application value of FQ-PCR(fluorogenic quantitative polymerase chain reaction) for detecting HP DNA in human gastric mucosa.
前言： 目的：评估荧光定量PCR在检测胃粘膜上幽门螺杆菌的DNA中的应用价值。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To establish a method of high resolution DNA typing for HLA B40 cross reactive groups (CREG) in Chinese with polymerase chain reaction with sequence specific primers (PCR SSP).
目的采用顺序特异引物聚合酶链反应技术(PCRSSP)，建立汉族人群HLAB40交叉反应组高分辨度dna分型方法。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（ RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（ RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
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