Methods First derivative spectrophotometry was used.

方法一阶导数分光光度法。

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Method: First derivative spectrophotometry was used.

方法：用一阶导数光谱法测定。

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METHODS: The first order derivative spectrophotometry was used.

方法：采用一阶导数分光光度法。

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METHODS: The first order derivative spectrophotometry was used.

方法：采用一阶导数分光光度法。

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