Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBV DNA ，综合分析各检测值对肝胃不和证积分的意义。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
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