ObjectiveTo study clinical meaning of new DNA chip technique in detecting the mutation of HBV-DNA , and to offer testimony for clinical diagnosis and treatment.
应用新型基因芯片研究乙肝病毒多点基因变异在慢性乙肝患者中的意义，为临床诊断和治疗提供依据。
Objective To study clinical significance of DNA chip technique in detecting the mutation of HBV-DNA ORF, and to offer testimony for clinical diagnosis and treatment.
目的应用新型基因芯片研究乙肝病毒多点基因变异在慢性乙肝患者中的意义，为临床诊断和治疗提供依据。
Objective To study clinical meaning of DNA chip technique in detecting the mutation of HBV-DNA ORF, and to offer testimony for clinical diagnosis and treatment.
目的研究HBV- DNA多点基因变异在慢性乙肝中的临床意义，为临床诊断和治疗提供依据。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
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