Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
方法采用聚合酶链反应-限制性片段长度多态性分析(PCR - RFLP)检测136例HBV DNA阳性的上海籍HBV感染者的基因型。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.
目的探讨荧光定量聚合酶链反应(FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒(HBV)敏感性的差异。
Objective: to develop an internal quality control substance of HBV-DNA by real time quantitative PCR and to evaluate its clinical value.
目的：制备h BV - DNA荧光定量pcr检测的室内质控物，并对其应用价值进行初步评价。
To investigate the change of HBV DNA in children with hepatitis B. HBVM and HBV DNA were detected by microparticle enzyme immunoassay (MEIA) and PCR respectively.
了解小儿乙型肝炎病毒(HBV DNA)复制水平的变化及其临床意义。采用微粒子酶免分析法(MEIA)检测HBVM，荧光定量pcr法检h BV DNA。
Objective To investigate the clinical value of endpoint detection of HBV-DNA by means of fluorescent quantitive PCR.
目的运用终点法荧光定量pcr检测HBV - DNA，探讨其临床应用价值。
CONCLUSIONS HBV DNA extracted from clinical samples were directly detected using PNA biosensor and PCR amplification was successfully bypassed.
结论利用肽核酸生物传感器成功地绕过了PCR扩增而直接检测出了临床标本中的HBV基因组dna。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBV DNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBV DNA ，综合分析各检测值对肝胃不和证积分的意义。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBV DNA、乙肝标志物及alt的变化情况。
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