If your gene goes in, you've interrupted the gene for antibiotic resistance and those new organisms aren't going to be resistant to antibiotic anymore.

如果基因进入了质粒,原本对抗生素有抗性的基因就被打断了,那些新细菌就不再对抗生素有抗性

耶鲁公开课 - 生物医学工程探索课程节选

One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.

一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性

耶鲁公开课 - 生物医学工程探索课程节选