This is another way to clone or make many copies of a gene of interest.

这是另一种克隆出许多,你感兴趣的基因的方法

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How we do that will be clear in a few minutes, but this polylinker as is described down here is a site where you can clone in genes.

马上你们就清楚这该怎么做了,这里所说的多克隆位点,就是你能把基因片段插进来的位点

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If I want to clone a gene from a human, if I want to clone the gene for human isulin, mean make many copies of the gene that's responsible for making insulin, I need to know whether there are introns there or not.

如果我想要从一个人类个体克隆基因,如果我想要克隆人类胰岛素基因,亦即我要把负责生成胰岛素的基因,复制许多个副本,我就得要知道哪些是内含子,哪些不是

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You're not just trying to clone the DNA, you're trying to also clone or make many copies of the protein.

你不仅想要复制DNA,更想要克隆或是制造,许许多多的蛋白质产物

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You have a fragment of some kind of DNA that you would like to clone or make many copies of.

你有了某种DNA的片段,想要克隆,复制它

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How did we put our gene fragments into this plasmid DNA in order to make multiple copies of it, or to clone the gene?

如何把基因片段整合到质粒DNA上,来获得大量拷贝,或者说来克隆基因呢

耶鲁公开课 - 生物医学工程探索课程节选