I talked about just not using viruses but using plasmid DNA together with lipids and that's another strategy for gene therapy.

这一部分我们不仅讲了病毒的应用,也讲了利用脂质体包裹的质粒DNA,基因治疗还有其它的策略

耶鲁公开课 - 生物医学工程探索课程节选

How did we put our gene fragments into this plasmid DNA in order to make multiple copies of it, or to clone the gene?

如何把基因片段整合到质粒DNA上,来获得大量拷贝,或者说来克隆基因呢

耶鲁公开课 - 生物医学工程探索课程节选

Well, plasmid DNA is neither of those.

然而,这两点质粒DNA都不具备

耶鲁公开课 - 生物医学工程探索课程节选

This is where - this is the region of the plasmid where we're going to insert the DNA that we're interested in.

这就是我们将在质粒上,插入我们感兴趣的DNA的位置

耶鲁公开课 - 生物医学工程探索课程节选

I'm going to start by talking about a very specific and interesting form of double stranded DNA called a plasmid and plasmids occur in nature.

我要从一种十分特殊,并有趣的双链DNA开始,这种双链DNA被称为质粒,它在自然界就存在

耶鲁公开课 - 生物医学工程探索课程节选

So bacteria that get an unloaded plasmid are going to have resistance to antibiotics.

得到无DNA片段质粒的细菌,就会对抗生素有抗性

耶鲁公开课 - 生物医学工程探索课程节选

Now, in your book, there's an example of plasmid where I've given you the exact sequence of nucleotides that makes up the whole double stranded DNA molecule.

现在在你们书里,有个质粒样本,我将组成这个双链DNA分子的,核苷酸序列写在这里了

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If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.

如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端

耶鲁公开课 - 生物医学工程探索课程节选

Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.

假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段

耶鲁公开课 - 生物医学工程探索课程节选

Now if I put them in contact with one another, the plasmid that's been opened and fragments of the DNA - special fragments that I've produced with the same restriction enzyme, they'll have the same sticky ends, they will naturally hybridize with one another.

如果我让它们互相接触,已经切开的质粒和DNA片段--,这DNA片段是我已用同种限制性内切酶,处理过的特别片段,这两者会有相同的粘性末端,它们会自然地相互杂交

耶鲁公开课 - 生物医学工程探索课程节选