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You then denature again, each one of these strands gets separated, primers bind, turn on the polymerase, a new strand is made.
然后你可以再次使产物链变性,使各链解旋,再结合引物,激活聚合酶,再生成新链
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Here's a way that you can do it in a test tube without using any cells by this process called Polymerase Chain Reaction.
但有一个方法可以让你在试管中完成,不用细胞,这个方法叫聚合酶链式反应
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The technique I want to talk about is one called Polymerase Chain Reaction.
我想讲的技术是聚合酶链式反应
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Polymerase needs a primer and that turns out to be important.
聚合酶需要引物,这点非常重要
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RNA polymerase is smart, it knows where it needs to go in order to make the copy of RNA that's required.
NA聚合酶很聪明,它知道该由什么位置介入,来转录所需要的RNA
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In this same way, this process of transcription which is occurring in cells throughout body all the time is made possible by a protein called RNA polymerase.
NA的合成也很类似,身体内每个细胞内,转录过程的进行都是在一种称作,RNA聚合酶的蛋白质催化下进行的
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I told you that that's driven by a protein called RNA polymerase.
此过程由称为RNA聚合酶的蛋白所介导
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The polymerase starts a process of replication of your DNA sequences.
聚合酶使DNA序列开始复制
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When they separate, the primers that you've added automatically bind through the process of hybridization, and then you turn up the temperature to the optimum for Taq polymerase and DNA synthesis starts.
当双链解旋后,加入的引物就会在杂交的步骤中自动,和解旋后的DNA结合,然后你再将温度升高至,Taq酶的最适温度,DNA就开始合成了
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Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.
请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制
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That DNA gets replicated to make many more copies of the viral DNA using host mechanisms, that is, often using the DNA polymerase which is naturally present in the host cell for its own replication.
病毒DNA进行复制,通常是利用在宿主细胞自身的有关物质,利用宿主细胞中天然存在的,DNA聚合酶来大量复制病毒DNA
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You add a special DNA polymerase called Taq polymerase And Taq polymerase is a polymerase that was identified, a DNA polymerase that was identified from an organism that lives in regions of the earth that are constantly at high temperature.
然后你加进一种特殊的,被称为Taq的DNA聚合酶,Taq聚合酶是已被鉴别出的聚合酶,这种DNA聚合酶是从一种生活在,地球上某些常年高温的区域中的,生物体内发现的
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A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.
引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的
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Synthesis always occurs in one direction and that makes sense to you now because you know there's a directionality and the chemistry is different going one way than the other and this DNA polymerase only works on the chemistry going in one direction.
NA复制过程是单向的,现在你们该明白原因了,因为DNA单链有方向性,DNA在两个方向上化学性质不同,DNA聚合酶,只在一个方向上起作用
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In order to have DNA you have to make DNA and your cells are continually making DNA inside your body, through a process of DNA synthesis and that synthesis is occurring because of the presence of an enzyme, a protein called DNA polymerase.
为了使每个细胞都具有DNA,身体通过一个叫做DNA合成的过程,不停的生产DNA,这个合成过程是在,一种酶的催化下进行的,这种酶叫做DNA聚合酶
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It operates in a similar fashion to DNA polymerase in that it denatures locally or opens up the double stranded DNA, but it's different in that it creates a new polymer from the DNA template in the language of RNA, using RNA nucleotides and not DNA nucleotides.
它的作用机制类似于DNA聚合酶,在局部使DNA双链变性,解旋,但不同的是,它合成了一个基于DNA模板的,使用RNA编码语言的新聚合物,即使用核糖核苷而非脱氧核糖核苷
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