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It has to have an origin of replication, which we talked about last time, which is compatible with the cells.
所以质粒必须带有复制起点,我们上次讲过,该起点要与受体细胞匹配
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So having a place that the cell knows - where the cell knows how to replicate is important and so plasmids have an origin of replication.
所以得有一个细胞能识别的区域,能使细胞知道,怎样来复制 这很重要,因此质粒上带有一个复制起点
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Well, of course it's a replication of B.
这里当然就是重复的B
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Hopefully their immune systems will respond to it like the real virus but it won't be capable of replication because we've chemically cross linked it so it can't go through its life cycle.
人体的免疫系统,就会对该病毒产生免疫应答,但灭活的病毒不能复制,因为我们已经对它做了化学处理,使它无法进入生命周期
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The polymerase starts a process of replication of your DNA sequences.
聚合酶使DNA序列开始复制
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That's synthesis and that has to happen in order for cells to replicate and cell replication is happening in your body all the time.
这个DNA合成过程是,细胞复制中不可或缺的部分,细胞复制时刻不停的在你身体里进行着
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This region here of this particular plasmid is called the ori or origin of replication.
质粒上的这个区域叫,ori,或者叫复制起点
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The result of this process if this replication went down the whole length of the DNA would be to form two identical, double stranded DNA molecules.
如果复制过程在整条链上持续进行的话,将生成两条完全一样的,双链DNA分子
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What's shown here is what's called a replication fork in DNA that's undergoing synthesis.
这里展示的是在DNA合成过程中,形成的DNA复制叉
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Remember we talked about how DNA replicates itself and that there are enzymes, DNA polymerase that bind to the double stranded DNA, separate it, denature it locally, and then start the process of replication.
请记住DNA复制必须有酶的参与,DNA聚合酶结合在双链DNA上,打开双链并原位变性,然后才开始复制
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A primer is a short RNA sequence or DNA sequence that gets sort of the process of replication jump started, and that's just because of the biological properties of DNA polymerase that that primer's needed.
引物是一段短的RNA或者DNA序列,引物是整个复制的起点,因为DNA聚合酶的,生物特性,引物在复制过程是必不可少的
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That DNA gets replicated to make many more copies of the viral DNA using host mechanisms, that is, often using the DNA polymerase which is naturally present in the host cell for its own replication.
病毒DNA进行复制,通常是利用在宿主细胞自身的有关物质,利用宿主细胞中天然存在的,DNA聚合酶来大量复制病毒DNA
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Certain segments of DNA are being converted into RNA, and whereas in replication, you have to copy the whole genome, the whole - all of the chromosomes, all of the DNA contained in the chromosomes of the cell in order to completely replicate it; transcription only works on particular sequences of DNA.
特定的DNA序列被转录为RNA,在复制的过程中,整条染色体都被复制,包括其中的全部DNA序列,都被分毫不差地复制下来,以保证复制的完整性,而转录过程只发生在特定的DNA序列上
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