If your gene goes in, you've interrupted the gene for antibiotic resistance and those new organisms aren't going to be resistant to antibiotic anymore.
如果基因进入了质粒,原本对抗生素有抗性的基因就被打断了,那些新细菌就不再对抗生素有抗性
One way that you can look for that gene that you want is by making the cut in your plasmid inside of a gene that encodes for some property like resistance to an antibiotic.
一种找到成功重组质粒的方式,是在质粒控制某种特性的编码区域切割,例如,对抗生素的抗性
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