The second issue is that the gene vector, in this case the plasmid, has to be compatible with the cells that you're trying to express the gene in.

接下来的问题是如何选择合适的基因载体,在这里用到的,质粒,必须与要用来表达基因的,受体细胞匹配

耶鲁公开课 - 生物医学工程探索课程节选

In fact, is doing some work with Professor Corbin and me jointly on the issue of food and addiction, and she'll be doing some lecturing on that later in the class.

事实上,她正在和柯宾教授还有我,一起研究食物成瘾问题,她之后会就这个话题给大家做一些演讲

耶鲁公开课 - 关于食物的心理学、生物学和政治学课程节选

This becomes a very, very large issue because the notion that there should be a real connection between citizenship in the full sense and military performance is totally a Greek idea--I mean, the Greeks just totally accept that idea.

这是一个很大的问题,因为完全意义上的公民,和参与军事行动是相联系的,这个观念完全是希腊式的,我指的是,被希腊人广泛接受的观念

耶鲁公开课 - 古希腊历史简介课程节选

It's still--they can make losses for a long time before they're in trouble, so presumably there won't be a problem, but in principle there could be and so that's the kind of issue that we have now.

在陷入困境之前的很长一段时间内,他们可以承受亏损,也可以假设不会有任何问题发生,但是原则上讲是有可能会出问题的,这就是我们面临的现状

耶鲁公开课 - 金融市场课程节选