They grew the virus in monkey cell cultures, they purified the virus because you got to get all the other stuff from the cells that you're growing it in a way, they inactivated it by treating it with formalin which is just a formula-- it's just a mixture of Formaldehyde; Formaldehyde cross links proteins.

研究人员将病毒种植在猴细胞内,进行培养,并提纯病毒,在某种程度上,通过这种方法,可以得到所有类型的病毒,研究人员用福尔马林,处理病毒,使其灭活,福尔马林就是甲醛的水溶液,甲醛可以使蛋白质发生交联反应

耶鲁公开课 - 生物医学工程探索课程节选

The Salk Vaccine is also called the Killed Polio Vaccine, and some people call it KPV, also sometimes called Inactivated Polio Vaccine, IPV, but this is the vaccine I just talked about produced by Salk.

索尔克氏疫苗也叫灭活脊髓灰质炎疫苗,有人将其简称为KPV 有时也被称作,失活脊髓灰质炎疫苗,简称IPV,这个疫苗由索尔克研发,刚才讲过他

耶鲁公开课 - 生物医学工程探索课程节选