Again, these plasmids occur naturally in nature; they were discovered particularly in micro-organisms have plasmids that confer biological properties onto them.
此外,质粒存在于自然界中,质粒是在微生物中发现的,它们也赋予了微生物一些生物学特性
Now most diabetics take human insulin made, not in humans, but made in micro-organisms that are growing in a manufacturing facility.
而现在,多数糖尿病患者用人的胰岛素,不是来自人体,而是来自工厂的微生物车间
You know, even some goes pass you on the street and you don't smile ostensibly you don't smile-- there are certain micro-muscles in your face that are moving that actually make you feel better.
即使路人与你擦身而过时,你没笑,表面上你没有笑-,但你面部的细微肌肉,会收缩,让你感觉更好。
You take that plasmid; you put in the micro-organism you're going to make many,many copies of that plasmid.
你把一个质粒放进微生物体内,你就会得到,很多很多质粒的拷贝
This restriction enzyme was found in a natural source, it was found in a micro-organism called E.coli.
这个限制性内切酶源于自然界,是从名为大肠杆菌的微生物中发现的
That is a way of putting a cloned gene into a micro-organism and then using that micro-organism as sort of a factory for the protein.
这方法把克隆基因植入微生物之后,将其作为某种形式的蛋白质工厂
Antibiotics are chemicals, usually small organic molecules that will kill micro-organisms like bacteria.
抗生素是一类化学物质,它们通常是小分子有机物,能够杀死细菌这样的微生物
If a cell has a gene that makes it resistant to Ampicillin, that means that that micro-organism can survive being exposed to this normally deadly chemical without dying.
如果细胞带有一种基因,能够让它对氨苄青霉素产生抗性,即使把它放在,有致死剂量化学物质的环境中,它也能存活下来
if you do that under the situation where you've put your plasmid into these micro-organisms then you're going to have little colonies that grow many copies of the bacterial cells.
如果你在质粒已经进入了微生物,的情况下培养它们,你就会得到许多小菌落,里面长有很多增殖出来的细菌
So being a micro-organism that got a plasmid that give you resistance to an antibiotic would be a good thing - that gave you resistance to something that naturally killed cells like you in your environment would be a good thing.
假如一个微生物,因含有质粒而具有抗生素抗性,这实在是一桩美事--,在同样的环境下,普通细胞会被杀死,而你们这些具有抗性的细胞却安然无恙,这实在太棒了
It's totally synthetic, there's no micro-organism involved in making the DNA for you, You can do it very rapidly in a laboratory, and fairly inexpensively now.
完全是复制的过程,没有任何微生物参与制造DNA,你可以在实验室里迅速地进行,费用现在也相当便宜了
The promoter in this case is called the lac promoter and normally in micro-organisms that lac promoter is used to produce a gene called lacZ which makes a protein called beta-galactosidase.
在这个例子中的启动子称作lac启动子,通常在微生物中,lac启动子是用来生成,一段叫lacZ的基因,这段基因,能制造一种蛋白叫β-半乳糖苷酶
Taq stands for thermos aquaticus, it's a marine micro-organism that lives near these hydrothermal events in the bottom of the sea and they live under very high temperatures and pressures all the time.
aq是"水生栖热菌"的简称,那是一种生活在靠近深海热液活动区的,海底微生物,它们长期生活在高温高压的环境中
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