If your gene goes in, you've interrupted the gene for antibiotic resistance and those new organisms aren't going to be resistant to antibiotic anymore.
如果基因进入了质粒,原本对抗生素有抗性的基因就被打断了,那些新细菌就不再对抗生素有抗性
And in general, when people devote a lot of energy or money or expense to something, they are extraordinarily resistant to having it proven wrong.
一般来说,当人们投入的精力,金钱或成本越多时,他们就越不会相信自己是错的。
If a cell has a gene that makes it resistant to Ampicillin, that means that that micro-organism can survive being exposed to this normally deadly chemical without dying.
如果细胞带有一种基因,能够让它对氨苄青霉素产生抗性,即使把它放在,有致死剂量化学物质的环境中,它也能存活下来
And people are often resistant to that.
但是人们经常不太喜欢这么做。
Well, let's try; let's be a little resistant here to this.
好的,让我们试一下,我们耐心一点。
For one thing, the brain is highly resistant to damage.
一方面,大脑并不容易受到损伤
If you selected the cells right than the only ones that have that resistance to Ampicillin are the ones that successfully got your plasmid and are using this Ampicillin resistant gene.
如果你选对了细胞,那么对氨苄青霉素有抗性的就是,成功得到了质粒并表达了基因的细胞
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