In this case, this particular restriction enzyme cuts symmetrically like this, but not at the same point.

在我们这种情况下,这个限制性内切酶总是这样对称地切割,但两条链切割的位点不一样

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If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.

而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对

耶鲁公开课 - 生物医学工程探索课程节选