If I want to clone a gene from a human, if I want to clone the gene for human isulin, mean make many copies of the gene that's responsible for making insulin, I need to know whether there are introns there or not.

如果我想要从一个人类个体克隆基因,如果我想要克隆人类胰岛素基因,亦即我要把负责生成胰岛素的基因,复制许多个副本,我就得要知道哪些是内含子,哪些不是

耶鲁公开课 - 生物医学工程探索课程节选

You're not just trying to clone the DNA, you're trying to also clone or make many copies of the protein.

你不仅想要复制DNA,更想要克隆或是制造,许许多多的蛋白质产物

耶鲁公开课 - 生物医学工程探索课程节选

How did we put our gene fragments into this plasmid DNA in order to make multiple copies of it, or to clone the gene?

如何把基因片段整合到质粒DNA上,来获得大量拷贝,或者说来克隆基因呢

耶鲁公开课 - 生物医学工程探索课程节选