If I'm going to make insulin from this I have to know that I've got the introns spliced out correctly.

如果我要用这些来生成胰岛素,我就得知道是不是把内含子都准确剔除了

耶鲁公开课 - 生物医学工程探索课程节选

The regions that are necessary for describing what the protein is like are called exons, the regions that are not are called introns.

因此我们把编码蛋白质的区域,称为外显子,而把不参与编码蛋白的区域称为内含子

耶鲁公开课 - 生物医学工程探索课程节选

There are encoding regions called exons and those are interrupted by non-coding regions called introns.

编码蛋白质的部分叫外显子,外显子被不编码蛋白质的内含子打断

耶鲁公开课 - 生物医学工程探索课程节选

The introns are gone now.

内含子没有了

耶鲁公开课 - 生物医学工程探索课程节选

If I want to clone a gene from a human, if I want to clone the gene for human isulin, mean make many copies of the gene that's responsible for making insulin, I need to know whether there are introns there or not.

如果我想要从一个人类个体克隆基因,如果我想要克隆人类胰岛素基因,亦即我要把负责生成胰岛素的基因,复制许多个副本,我就得要知道哪些是内含子,哪些不是

耶鲁公开课 - 生物医学工程探索课程节选

That messenger RNA that's being used has already gone through the RNA splicing mechanism and so the introns have already been removed.

这个mRNA,已经经过了RNA剪接机制的剪接,切去了内含子

耶鲁公开课 - 生物医学工程探索课程节选

If I cut up just DNA from the chromosome, what's called genomic DNA, then I'm going to have both exons and introns within the fragments that I create.

如果我切割的恰好是染色体上的,基因组DNA,那我切成的片段上,就会同时有外显子和内含子

耶鲁公开课 - 生物医学工程探索课程节选

One is the processing has already been done so the introns are already out, so you don't have to figure out what's exon and what's the intron, it's already done for you.

一是DNA已经过处理,内含子已经没有了,你不用分辨哪些是外显子哪些是内含子,这些都已经帮你做好了

耶鲁公开课 - 生物医学工程探索课程节选