Sometimes a blunt cut is useful if you want to sort of grow a sticky end of your choice on it.

这时候钝切口很有用,尤其在你想获得选定的粘性末端的时候

耶鲁公开课 - 生物医学工程探索课程节选

When it cuts it leaves sticky ends or un-base paired single stranded regions on each end of the part its cut and that's just a property of many restriction enzymes; not all, some cut blunt,just right down the middle.

它切割后会留下两个粘性末端,或者未配对的单链部分,这是许多限制性内切酶的特性,但不是全部,有些限制性内切酶是从中间钝切的

耶鲁公开课 - 生物医学工程探索课程节选