Isolated Microspore culture is one of the methods to obtain haploid.
游离小孢子培养技术是获得大白菜单倍体植株的有效途径之一。
The best development period for microspore culture is monokaryotic stage;
小孢子离体培养的最佳发育时期是单核期；
Rape have established regeneration system of cotyledons, hypocotyls, protoplasts and microspore culture.
油菜已经建立了子叶、胚轴、原生质体及小孢子培养的再生体系。
We obtain haploid by isolated microspore culture, and the pure-line is very useful in Purple Flowering Stalks breeding.
利用游离小孢子培养技术获得单倍体，所获纯合系在育种上有较高利用价值。
Tissue culture, such as microspore culture and embryo technique was widely developed in rapeseed breeding and fundamental studies.
小孢子技术、体细胞原生质体融合技术等组织培养手段已广泛应用于油菜育种及基础性研究。
The amount of pollen plants regeneration from a bud in the isolated microspore culture is higher slightly than that in pollen culture.
而每花蕾成苗数则游离小孢子培养略高于花药培养。
The effect isn't notable on embryoid induction for "Y009", but it can keep materials fresh for isolated microspore culture temporarily.
该影响在“Y009”上表现不显著，但可以暂时保存材料而不降低培养效果。
This study focus on selection of disease-resistant restorer lines through two approaches of microspore culture and marker assisted selection.
本研究以小孢子培养和分子标记辅助选择两种途径来选育抗病的恢复系。
In recent 20 years with more studies of rapeseed microspore culture a perfect regenerative system of rapeseed microspore culture has been set up.
近20年，油菜小孢子培养已得到深入的研究，并建立了完善的油菜小孢子再生体系。
The efficiency of regeneration of pollen plants with the pollen embryo inducement in pollen culture is higher greatly than that of isolated microspore culture.
花药培养的花粉胚植株再生率要明显高于游离小孢子培养。
Also, the progresses in researches of barley microspore culture, cell suspension culture, protoplast culture and protoplast fusion are briefly introduced in this paper.
并对大麦小孢子培养，细胞悬浮培养，原生质体培养和原生质体融合研究的最新进展作了简要介绍。
The procedure of isolated microspore culture with higher regeneration was established by the technique optimization and the platform of maize DH breeding was developed.
通过技术优化，建立玉米小孢子离体培养高频再生技术程序，在此基础上构建了玉米dh育种技术平台。
Stable-lines could be developed through platform of microspore culture derived DH (double haploid) breeding technique within two years and stable variety could be produced within 4 years.
利用大麦小孢子加倍单倍体育种技术平台，二年内就可获得种性稳定的株系，四年内即可参加新品种区试。
The results showed that genotype plays a decisive role on informing embryoid in microspore culture, the induction frequency of embryoids significantly difference among different genotypes As no.
结果表明：供体植株基因型是影响小孢子发生胚状体的关键因素，不同材料之间的诱导频率差异显著；
Using field-grown plants of lines and F1-F2 hybrids of barley as experimental materials, a procedure of microspore culture in vitro with high regeneration was established by technique optimization.
以大麦F1、F2代为供体材料，通过技术优化，建成了大麦小孢子培养高频再生技术程序。
Improving microspores embryogenic ability and plantlet regeneration ability from microspore-derived embryos is the key of isolated microspores culture technique.
胚胎发生能力和再生植株转化率是游离小孢子培养技术的关键问题。
Improving microspores embryogenic ability and plantlet regeneration ability from microspore-derived embryos is the key of isolated microspores culture technique.
胚胎发生能力和再生植株转化率是游离小孢子培养技术的关键问题。
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