The mobile phase consisted acetonitrile - water( 1:4).
以乙腈-水（1：4）为流动相；
The conditions for extraction and mobile phase were optimized.
优化不同性质样品的提取条件及测定的流动相。
The sample is transported through the column by the mobile phase .
试样由移动相带着穿过色谱柱。
In some cases, the sample may be diluted with mobile phase to improve the peakshape.
在一些情况下，为了改善峰形，样品溶液可以用流动相稀释。
It is described that effect of resin type, mobile phase flow and temperature on separation.
探讨了树脂类型、洗脱剂流量和酸度及层析温度等对分离的影响。
With the different state phase and mobile phase, the separation of monosaccharide was researched.
在不同的固定相和流动相条件下，对单糖的分离情况进行了分析。
The title constants were determined by reverse-phase HPLC on an ODS column with methanol as mobile phase.
提出用反相高效液相色谱法测定文题常数。
Additionally, enthalpy and entropy both contributed to enantioseparation when mobile phase was acetonitrile.
流动相为纯乙腈时，焓和熵对对映体的拆分均有贡献。
The detection wavelength, sample preparation and the optimization of mobile phase constitution were discussed.
讨论了检测波长的选择和样品的制备，优化了流动相的组成。
The composition of mobile phase has different influence on the elution range and ability to the two types of solutes.
流动相组成对这两种类型溶质的洗脱范围及洗脱能力的影响不同。
There are many advantages which the ordinary CEC method doesnt have by using the pure organic solvent as mobile phase.
它以纯有机溶剂作电色谱流动相，具有许多水溶液电色谱所不能比拟的优点。
In adsorption chromatography, solute molecules of the mobile phase undergo many successive adsorptions and desorptions.
在吸附色谱中，流动相的溶质分子进行多次连续的吸附和脱附。
Objective: to establish an HPLC method for the resolution of metoprolol enantiomer using chiral mobile phase additives.
目的：建立hplc手性流动相添加剂法拆分美托洛尔对映体的方法。
Methods Under linear scale up mode, overlapping separation range map was used to optimize the composition of mobile phase.
方法在线性放大模式下，使用重叠分辨图法对流动相组成进行优化调节。
NH2 column was used with the mobile phase of acetonitrile-water (75:25). The differential refractive index detector was used.
采用氨基键合硅胶柱，以乙腈-水(75∶25)为流动相，使用示差折光检测器。
The validity of the method is verified through the selection of optimal operating condition in binary and ternary mobile phase systems.
通过对反相色谱二元和三元体系操作参数的优化选择，确证了方法的可靠性。
The effect of charge-transfer(CT) interaction between the solute molecules in mobile phase on chromatographic process was investigated.
本文从溶质分子间的电荷转移相互作用，讨论了溶质浓度对色谱过程的影响。
Chloroform - tetrahydrofuran - water (500:500:8) was used as mobile phase for the gradient elution of other strongly retained substances.
继用氯仿-四氢呋喃-水(500：500：8)为流动相，梯度洗脱其他强保留组分。
METHODS: Fixing other conditions than the composition and ratio of the mobile phase, estimate the optimum according to the separation results.
方法：固定其他色谱条件，改变流动相组成与配比，以分离结果的优劣优化流动相。
Method: Same mobile phase and detection wavelength were selected and suitable precipitating agent was used. The HPLC conditions were optimized.
方法：选择相同的流动相和检测波长，采用适当的沉淀剂，对四种添加剂的检测条件进行了优化。
The influence of water proportion in mobile phase, flow rate of chromatography, derivatives of various kinds of alcohol were systematically examined.
考察了不同比例的乙醇水流动相、不同流速以及用不同醇类酯化对托品酸酯在手性色谱柱上的色谱行为。
Study on HPLC method for analyzing of trace pigments in ready-to-drink tea was conducted by using different eluants with acetonitrile as mobile phase.
以含乙腈的流动相作为洗脱剂，对茶饮料中微量色素物质的HPLC分析方法进行了研究。
The effects of temperature, flow rate and composition of mobile phase on retention, resolution, column efficiency and the pressure of the system were examined.
考察了温度、流速和流动相组成对酚类样品的保留、分辨、柱效和系统压力的影响，探讨了酚类样品在聚合物柱上的热力学行为。
The influence of types and concentration of ionic liquids, detection wavelength, proportion of mobile phase, flow rate on the result of detection were discussed.
探讨了包括离子液体探针试剂的种类、用量、检测波长、流动相比例、流速等对检测效果的影响。
The influence of types and concentration of ionic liquids, detection wavelength, proportion of mobile phase, flow rate on the result of detection were discussed.
探讨了包括离子液体探针试剂的种类、用量、检测波长、流动相比例、流速等对检测效果的影响。
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