DNA, complementary; cloning, molecular; sequence analysis; gene expression.
互补；克隆分子；序列分析；基因表达。
In 1990s, molecular biological approach has led to a series of cloning and functional expression of ligand-gated P2X receptors and G-protein coupled P2Y receptors.
90年代，采用分子生物学手段，一系列配体门控的P2 X受体及G蛋白耦联的P 2y受体被克隆及功能表达。
Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
Purpose Molecular cloning and expression of pro nattokinase gene with e.
目的构建纳豆激酶原基因克隆，实现其在大肠杆菌中的表达。
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