Basic work for the system establishment about protoplast culture and physiological research.
系统建立的基础性工作：原生质体培养与生理研究。
This is the first report of plant regeneration from the protoplast culture of an indica rice.
这是迄今第一个由籼稻原生质体再生植株的报道。
The latest developments on protoplast culture and protoplast fusion are focused in this review.
本文综述了近年来世界各国果树原生质体培养和细胞融合的研究进展。
According to the target culture, tissue culture can be divided into organ, cell suspension and protoplast culture.
据所培养的对象，组织培养可分为器官、细胞悬浮和原生质培养。
It is reviewed on protoplast culture plant regeneration, somatic hybridization by protoplast, and protoplast as an acceptor of genetic engineering in this literature.
对甘薯原生质体培养植株再生、利用原生质进行体细胞杂交和作为遗传工程受体的研究进行了综述。
Also, the progresses in researches of barley microspore culture, cell suspension culture, protoplast culture and protoplast fusion are briefly introduced in this paper.
并对大麦小孢子培养，细胞悬浮培养，原生质体培养和原生质体融合研究的最新进展作了简要介绍。
Recently, the protoplast culture of medicinal plants has mace great progress in the saltation of high productive cell lines, somatic cell hybridization and gene engineering.
目前，原生质体培养在药用植物高产细胞系的筛选、体细胞杂交和基因工程方面已取得了长足的进展。
It's organ culture and protoplast culture is important basic technique of rapid clonal propagation, selection of imitative plants, somatic hybridization and genetic transformation.
红麻的器官培养和原生质体培养是品种快繁、变异体筛选以及体细胞杂交和遗传转化等生物技术育种的基础。
Therefore, it is significant to further study protoplast culture and fusion which could create new germplasm and be useful to genetic improvements of cultivars and rootstocks in apple.
进一步开展苹果原生质体培养融合技术的研究，对于创造新种质材料，促进原生质体技术在苹果品种和砧木遗传改良中的应用将具有重要的意义。
The formation of the system of protoplast culture has an important significance in various kinds of genetic operations and in utilizing biological technology to cultivate new varieties.
原生质体培养体系的建立对各种遗传操作和利用生物技术培育新品种具有重要意义。
Results are as follows: 1. Axillary buds culture and callus differentiation were studied in this paper, which provide experience for in vitro chromosome doubling and protoplast culture techniques.
主要研究结果如下：1。研究了小青杨单芽茎段培养和愈伤组织再生技术，为进一步开展离体叶片细胞染色体加倍以及原生质体培养奠定了基础。
The isolation, culture and fusion technology of plant protoplast have been developing rapidly over the past twenty years.
对原生质体的分离、培养、融合及植株再生方面进行了简要的论述。
The results showed that the cellobiose medium and double layer culture method were most suitable for protoplast regeneration of Ganoderma lucidum.
结果表明，纤维二糖培养基及双层培养法比较适合灵芝原生质体再生。
The plant regeneration systems of some turfgrass species have been established perfectly through callus culture, cell suspension culture, and protoplast suspension culture.
通过愈伤组织培养、悬浮细胞培养和原生质体培养方法对草坪草的一些种已建立较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture, suspension culture, and protoplast suspension culture.
通过愈伤组织培养、悬浮细胞培养、原生质体培养等手段，对早熟禾属、黑麦草属、结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。
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