The strategies of quality improvement and theuses of SDS-PAGE in wheat breeding programmes were discussed.
本文还对品质改良策略和SDS—PAGE在育种实践中的应用进行了讨论。
According to the analysis of SDS-PAGE, the bands of the graft subunits decreased and the glycosyl bands were seen more clearly with the degree of graft (DG) increasing.
聚丙烯凝胶电泳(SDS - PAGE)分析结果表明,随着接枝度(DG)的增加,接枝物的亚基谱带逐渐减少,糖基谱带变得明显。
Analyse the protein difference of purification process Through SDS-PAGE.
PAGE分析纯化过程中蛋白质组成的差异。
Then, antigen was prepared by conjugation of hapten with carrier protein by carbodiimide (EDPC) method and was identified by UV spectroscopy and SDS-PAGE.
然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原,采用紫外扫描及SDS - PAGE鉴定。
The types, distribution and histochemistry characters of mucous cells of Meretrix meretrix Linnaeus were studied by histological and histochemical methods and SDS-PAGE electrophoresis.
运用组织学、组织化学及聚丙烯酰胺凝胶电泳技术,研究了文蛤粘液细胞的类型、分布及组化特性。
Results: (1) Molecular quantity of the purified flagellin was about 65kd by SDS-PAGE electrophoresis.
实验结果:(1)纯化的鞭毛蛋白经sDS - PAGE电泳,分子量约为65kd。
The 7s and 11s globulin content of 5 soybean varieties grown in 11 locations of Heilongjiang province, Jilin province and Liaoning province were analysed with the line gradient SDS-PAGE.
利用线性梯度SDS - PAGE方法分析东北地区(黑龙江省、吉林省和辽宁省)11个地点的5个大豆品种的7s和11s球蛋白含量。
The fraction of vegetative storage protein(VSP) in different parts of Ginkgo biloba and its annual dynamic changing rules were studied by the technology of SDS-PAGE and microscope.
采用SDS-聚丙烯酰胺凝胶电泳技术和电子显微技术,对银杏不同部位的营养贮藏蛋白质组分及其动态变化规律进行了研究。
Every collected peak was identified by SDS-PAGE after purified with gel chromatography by fast protein liquid chromatography system.
结合物经快速蛋白质液相层析系统用凝胶色谱柱纯化后,用SDS PAGE鉴定各收集峰。
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.
重组人脑源性神经营养因子(BDNF)在大肠杆菌中表达,以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96%。
Method: Use the method of indirect immunofluorescence, mice cross protection test and SDS -page to determine the serum type, the toxicity and the protein atlas of the isolated strains.
方法:采用间接免疫荧光法、小鼠保护试验以及SDS -聚丙烯胺电泳法,分别测定分离株的血清类型、毒力和蛋白图谱。
SDS-PAGE and scanning monitor were used to detect expression efficiency and analyze the solubility of protein.
采用SDS - PAGE及岛津薄层扫描分析仪进行表达效率检测、蛋白可溶性的扫描分析。
The high molecular weight glutenin subunits and their encoding genes in Aegilops variables were characterized by SDS-PAGE and molecular cloning.
利用SDS-PAGE和分子克隆方法研究了易变山羊草的高分子量谷蛋白亚基及其基因。
The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE.
所得极低密度脂蛋白(VLDL)及低密度脂蛋白(LDL)经琼脂糖电泳、聚丙烯酰胺电泳(PAGE)及载脂蛋白SDS-聚丙烯酰胺电泳(SDS-PAGE)鉴定纯度良好。
Method Type I collagen was attained from rat tail tendon with method of acid extraction, then undergone analysis and identification using ultraviolet spectroscopy, SDS-PAGE and IEF.
方法采用酸提法从鼠尾肌腱中提取I型胶原蛋白,并用紫外扫描法、SDS - PAGE和IEF进行分析和鉴别。
SDS-PAGE is an effective method for protein analysis, comparison and characteristic identification.
PAGE是蛋白质分析、比较和特性鉴定的有效方法。
SDS-PAGE was used to analyze proteolysis of dry-salted duck samples of different processing phases and the free-amino acid(FAA) were tested as well.
用SDS-PAGE电泳分析蛋白质的降解规律,同时比较加工初期及末期游离氨基酸的变化,并以不接菌样品为对照。
It was shown as a single band in SDS-PAGE. The molecular weight of lactoperoxidase was 75035d.
经SDS - PAGE测定,分离出的乳过氧化物酶显示为单一区带,相对分子质量为75035d。
Methods Casein was hydrolyzed by trysin and chyomtrysin together. The enzymatic hydrolysis was filtrated by ultrafiltrate membrane, and analyzed by SDS-PAGE.
方法将酪蛋白酶解,其酶解产物经超滤膜过滤,用十二烷基磺酸钠——聚丙烯酰铵凝胶电泳(SDS-PAGE)方法分析其酶解情况。
The 190kd protein of motor nerve was isolated from the anterior roots of human spinal cord by the SDS-PAGE.
采用SDS凝胶电泳方法从人脊神经前根中分离出运动神经元特有的蛋白组份190KD蛋白。
Methods nuclear matrix proteins were extracted under high salt extraction procedures, and changes of nuclear matrix proteins were analysed by SDS PAGE and two dimensional (2d) electrophoresis.
方法应用高盐提取法抽提细胞核基质蛋白,SDS PAGE、双相电泳(2d)法分析白血病细胞与正常骨髓细胞核基质蛋白的变化。
Methods The soluble antigens of the parasites were analysed by means of SDS PAGE and two dimensional gel electrophoresis.
方法用SDS- PAGE和双向电泳方法,对日本血吸虫两性成虫全虫可溶性抗原进行分析。
The subunit molecular weight was determined as 32600 by SDS-PAGE.
用SDS-聚丙烯酰胺凝胶电泳测定亚基分子量为32600。
On SDS-polyacrylamide gel electrophoresis (SDS-PAGE)the eluate from the column with the McAb coupled showed a single band, corresponding to the elution peak.
聚丙烯酰胺凝胶电泳显示单抗偶联柱层析洗脱物仅有单一区带与洗脱峰对应;
After SDS-PAGE, the recovery of CP added the same volume of incomplete Freund Adjuvant emulsified completely, injected rabbits by subcutaneous injection.
PAGE电泳后,将表达的CP蛋白切胶回收,研磨成粉后加入等体积的福氏不完全佐剂,乳化制备成抗原。
SDS-PAGE showed that OBPC and the four solubility fractions had different polypeptide compositions.
PAGE的结果表明OBPC及各蛋白组分有不同的分子组成。
The obtained proteins were checked for the purity through SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
用十二烷基硫酸钠-凝胶电泳(SDS - PAGE)和二维凝胶电泳鉴定蛋白质纯度。
Results SDS PAGE and thin layer scanning showed that the expressed product contained about 6.4% of total somatic protein.
结果经SDS PAGE和薄层扫描分析表明,外源蛋白表达量占菌体裂解蛋白总量的6 . 4 %。
Results SDS PAGE and thin layer scanning showed that the expressed product contained about 6.4% of total somatic protein.
结果经SDS PAGE和薄层扫描分析表明,外源蛋白表达量占菌体裂解蛋白总量的6 . 4 %。
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