At last it was measured by SDS-PAGE, Enzyme-linked immunosorbent assay and HPLC.
用SDS -聚丙烯酰胺凝胶电泳、酶联免疫法和高效液相测纯度。
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.
通过SDS - PAGE检测融合蛋白的纯度,采用点杂交和纤维蛋白凝结活性检测分析,显示纯化产物具有较高生物活性。
SDS-PAGE was used to detect the purity of target protein. A higher bioactivity of purified products was shown by the determination of dot-blotting assay and fibrinogen-clotting analysis.
通过SDS - PAGE检测融合蛋白的纯度,采用点杂交和纤维蛋白凝结活性检测分析,显示纯化产物具有较高生物活性。
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