The positive clones were sequenced, and the sequences were analyzed by the BLAST program for homology search.
挑选阳性克隆进行测序并用BLAST软件进行同源性分析。
Three rounds of biopanning were carried out. The positive clones were randomly selected, detected and sequenced.
随机挑取噬菌体克隆，检测其特异性并进行序列分析；
The results showed that the positive clones screened by G418 were mouse fibroblast cells with high expression level of VEGF.
结果显示：经g 418筛选后的阳性细胞克隆，为高表达VEGF的鼠成纤维细胞模型。
The positive clones, of which the chromosomes were integrated with the defensin gene, were identified by the phenotype and PCR.
经表型筛选和PCR鉴定证明目的片段已稳定整合到酵母染色体基因组中。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。
The positive clones were sequenced and the sequence data were analyzed using Nucleotide BLAST software of NCBI and Expert Protein Analysis System of Swiss Institute of Bioinformatics.
通过互联网对测序获得的核苷酸序列进行同源性分析 ，并预测新基因编码蛋白质的结构与功能。
Positive clones were obtained by the selection inG418 conditioned culture.
经g 418条件培养筛选，获得阳性细胞克隆。
Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
以该融合蛋白为固相抗原，对噬菌体抗体库进行3轮淘筛，并对所获阳性克隆进行抗原结合活性测定和DNA序列测定。
A total of 141 positive clones were obtained by screening the SSH library.
通过筛选差减文库，共获得了141个克隆。
The genomic expression library was probed used the adsorbed-pooled sera, and 12 positive clones were obtained from 3000 postulating colonies by immunological hybridization.
用吸附后的血清通过菌落原位免疫杂交筛选该基因组表达文库，从3000个菌落中获得了12个阳性克隆。
The false positive clones were picked out by differential hybridization screen.
获得的阳性克隆进一步进行差异杂交筛选，去除假阳性。
This project not only increased the expression level of the exogenous gene, but also drastically reduced the labor strength of screening positive clones.
此设计方案不仅大大提高了外源基因的表达量，且极大地降低了筛选阳性克隆的劳动强度。
The pool positive phage clones after immune adsorption strongly recognized by the rabbit serum of the new model and the normal model, weakly recognized by the SEA immune rabbit serum.
在经SEA免疫兔血清吸附前，新模型兔血清来源的阳性噬菌体多克隆与SEA免疫兔血清呈阳性反应；
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
Among these techniques, the suppression subtractive hybridization (SSH) method has been shown to be quick and highly efficient, and it generates less false-positive clones.
在这些技术当中，抑制消减杂交(SSH)具有快速、高效、假阳性率低等特点。
The positive recombinant clones was sequenced and analysed. The results suggested that FnBPA D region gene was cloned successfully.
克隆产物经核酸序列测定后表明已成功克隆了目的基因。
DNA sequence assays showed that 5 of the 6 positive clones displayed the same peptide sequences.
DNA测序结果显示，所得到的6个噬菌体阳性克隆中，有5个阳性克隆递呈相同的氨基酸序列。
Some Positive clones were obtained. Meanwhile, the insert fragments were cloned and sequences were analyzed.
获得了一些有杂交信号的噬菌斑，并对目的片断进行克隆和序列分析。
Some Positive clones were obtained. Meanwhile, the insert fragments were cloned and sequences were analyzed.
获得了一些有杂交信号的噬菌斑，并对目的片断进行克隆和序列分析。
应用推荐