METHOD Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳技术。
Methods Whole-cell patch clamp technique was used.
方法采用全细胞膜片钳制技术。
Methods The whole-cell patch clamp with current clamp was used.
方法采用全细胞膜片钳技术，进行电流钳制。
Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.
方法：采用全细胞膜片钳技术，记录钠通道电流。
METHODS: Auditory brainstem responses (ABR) and whole-cell patch clamp techniques were used.
方法：应用听觉脑干诱发电位和全细胞膜片钳制技术。
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
METHODS: the technique of whole-cell patch clamp was used to record the chloride currents and to analyze the characteristics of the currents in CNE-2Z cells.
方法：采用全细胞膜片钳技术记录蟾蜍灵激活cne - 2 Z细胞膜电流并分析其电流特征。
The characterizations of voltage-activated potassium channels were studied on newborn bovine retinal neurons in long-term cultures with whole-cell patch clamp technique.
目的探讨体外培养不同时期新生小牛视网膜神经元电压门控钾离子通道的特性。
Whole-cell patch clamp technique was used to study the electrophysiological characteristics of nociceptive trigeminal ganglion (TG) neurons labeled by nuclear yellow (NY).
实验利用全细胞膜片钳技术研究了荧光染料核黄（NY）标记的支配牙髓的痛感觉神经元的电生理特征。
METHODS Patch clamp technique with whole cell configuration.
方法膜片钳制技术全细胞记录模式。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
METHODS The patch clamp whole cell recording technique was used.
方法采用膜片钳全细胞式记录技术。
Methods We used the improved enzymatic dissociation method and the patch clamp whole cell recording.
方法采用改进的酶解分离法分离单个细胞并经膜片钳全细胞记录法进行鉴定。
Methods the chloride channel currents were recorded in cultured A549 cells by using the whole-cell mode of patch clamp techniques.
方法在离体培养的A549细胞上，用膜片钳全细胞模式记录氯通道电流。
Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.
方法传代得到单个HERG-HEK细胞，应用全细胞膜片钳技术记录HERG-HEK细胞上的HERG钾电流和动力学曲线（激活、失活、复活和去活化）。
METHOD Whole cell patch clamp techniques.
方法全细胞膜片箝技术。
Results: (1) Whole-cell patch-clamp recording technique was established, and highly stable whole cell ion channels' current curves of normal ventricular myocytes were ploted.
结果：(1)建立了膜片钳全细胞记录技术平台，获得了高稳定性的正常心室肌细胞全细胞离子通道电流图。
Methods Effects of quinidine and verapamil on the alteration of action potential duration induced by aconitine were studied by use of the whole cell patch clamp techniques in rat ventricular myocytes.
方法应用全细胞膜片钳技术记录乌头碱对大鼠心肌细胞动作电位时程的作用，分别给予奎尼丁、维拉·帕米处理比较二者对动作电位时程的影响。
Methods: Patch clamp whole cell recording technique was used.
方法：膜片钳全细胞电流记录方法。
Methods: the whole cell patch clamp technology was used to record this current and study its change under the condition of hypoxia-reoxygenation model.
方法：运用全细胞膜片钳技术记录持续性钠电流，并观察其在缺氧-复氧模型下的变化。
Methods: The isolated neurons of dorsal root ganglion (DRG) were studied using the whole cell patch clamp technique in current clamp mode.
方法：应用全细胞膜片箝电流箝制技术，对急性分离的大鼠DRG细胞进行电流箝制。
Mechanical dissociation was used to obtain single primary spermatocytes from guinea pigs and iron channel currents in the primary spermatocytes were recorded by whole cell patch-clamp technique.
笔者应用机械分离获得豚鼠单个初级精母细胞，采用全细胞式膜片钳技术记录该细胞离子通道电流。
Using the whole-cell patch-clamp technique, clear evidence was provided for the existence of a hitherto unreported, voltage-dependent nonselective cation current (NSCC) in rabbit ventricular myocytes.
应用全细胞膜片钳制技术，我们首次报道了在兔的心室肌细胞存在一种电压依赖性的非特异性阳离子流（NSCC）。
The whole-cell patch-clamp recording technique was used to record the change of sodium channel current influenced by carvedilol.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
METHODS Voltage gated potassium outward current was recorded using whole cell patch clamp techniques in the cultured rat hippocampal neurons.
方法在培养的大鼠海马神经元上用膜片钳全细胞记录钾通道电流。
Method The whole cell patch clamp method was used.
方法应用全细胞膜片钳制技术。
The change of sodium currents (INa) induced by U50,488H was detected by whole-cell recording mode using patch clamp.
常规酶解法分离成年大鼠心室肌细胞，采用全细胞膜片钳技术观察U50，488H对钠电流的作用。
METHOD The whole cell patch clamp techniques were used.
方法采用全细胞膜片钳技术。
Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.
方法实验采用的方法是全细胞膜片钳技术，实验材料为急性分离的牛蛙背根神经节神经元。
METHODS: Whole cell patch-clamp recording techniques.
方法：全细胞膜片箝技术。
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