随后人GRP94启动子克隆了荧光素酶基因上游区且缺氧环境中活性增强。
The human GRP94 promoter was then cloned upstream of the luciferase gene and showed enhanced activity in hypoxic conditions.
通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。
The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.
研究者用基因工程的大肠埃希菌细胞生产荧光素酶作为AHL浓度函数。
The investigators used genetically engineered E. coli cells to produce luciferase as a function of AHL concentration.
方法采用荧光素酶报告基因方法。
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
对发现的突变，进行荧光素酶报告基因瞬时表达研究。
The reporter gene was used to study the mutation influencing the activity of promoter.
瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
以含雌激素反应元件的荧光素酶(ERE -LUC)为报告基因，通过检测荧光素酶活性来确定MDM2是否对ER有转录调节因子的作用。
The effect of MDM2 on the transcriptional activity of er was detected by the reporter gene containing estrogen responsive elements luciferase (ERE-LUC).
创伤后并发器官损害病人的荧光素酶报告基因活性显著高于无器官损害者，其死亡率也较高。
On the other hand, the activity of LUC was higher in the organ damagd patients than that in the non-organ damagd patients, with a high mortality rate.
荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。
Luciferase reporter gene technique plays an important role in high throughput screening.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
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