说明KSOM可以用于绵羊胚胎早期发育的无血清培养液。
This paper suggested that KSOM can be used as serum free medium for ovine embryo development in vitro.
方法用低血清培养液消化处理乳鼠肺组织进行单层细胞和组织块培养。
Methods the lung tissue cells of the new-born rats were treated by the low-serum culture medium, and the culture of the simple cell and the tissue blocks was conducted.
结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。
It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium.
结论应用“M”无血清培养液简化了雪旺细胞的培养技术，有效地控制了成纤维细胞的污染。
Conclusions With the application of the "m" serum - free medium, the technique of Schwann cell culture was simplified and the contamination of fibroblasts was effectively controlled.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶-EDTA消化传代。
Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The effects of fetal bovine serum (FBS) addition time, concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
结论用明胶包被培养皿，在培养液中加入肝素、内皮细胞生长因子及高浓度的胎牛血清可获得较纯的内皮细胞。
Conclusion the suitable condition for endothelial cell were use of gelatin coat culture dishes, and adding of heparin, endothelial growth factor and highly fetal serum into culture media.
取第3代骨髓基质细胞，以含胎牛血清的DMEM培养液培养消化后，计数细胞量，描绘细胞增殖曲线。
After 3 passages were obtained, and then digested by DMEM medium containing fetal calf serum, cell amount was counted and the proliferation curve of MSCs was depicted.
培养液中的白蛋白、血清可降低转染效率。
Albumin, serum in the culture medium decreased the transfection efficiency.
采用比色法检测血清、淋巴细胞培养液和肌肉组织中的NO和NOS。
Colorimetry was used to detect the levels of NO and NOS in the serum, lymphocytic culture liquid and muscular tissue.
细胞培养液中加入一定浓度硒可有效地减轻无血清培养引起的细胞损伤。
Selemum with given concentrations into cell cultures could effectively reduce the damages caused by serum free culture.
用不含血清和含0.5%血清的培养液直接饥饿指数生长期的成纤维细胞，细胞数量先是略有增长，然后逐渐下降。
After used medium in eluding 0.5% NCS and without NCS direct starvation, the Numbers of fibroblast first in creased slightly, then gradually decreased.
空白组加入含生理盐水血清的培养液，不加地塞米松。
The medium containing saline serum was added in the blank group, without dexamethasone.
在牛卵母细胞体外成熟培养过程中，本研究在同一种成熟培养液中，分别添加用0.3％PVP和10％FBS，检验PVP代血清培养是否可行。
During the bovine oocyte maturation, this research examined the possibility of serum-free maturation which 10%FBS was replaced with 0.3%PVP.
添加新生牛血清的KSOM与胚胎培养液G1/G2均有效克服昆白小鼠2-细胞阻滞，并能得到较高的囊胚率。
The KSOM medium with 10% NBS and medium G1/G2 were effective to overcome the 2-cell block, and the blastocysts rate of the two groups were high.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
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