Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene.
结果：与普通pcr相比，梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。
Aldosterone secretion is regulated largely at the level of expression of the final enzyme required for its biosynthesis, aldosterone synthase (CYP11B2).
醛固酮合酶(CYP11B2)是醛固酮生物合成最后一步的催化酶。醛固酮的分泌主要由醛固酮合酶的表达水平来调节。
Several genetic polymorphisms of the aldosterone synthase gene (CYP11B2), which may influence the plasma aldosterone levels, had been reported to influence the blood glucose levels.
醛固酮合成酶基因(CYP11B2)部分位点的多态性改变除导致血浆醛固酮水平发生改变外，还与血糖变化密切相关。
Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
Methods: Gradient PCR was employed in analysis of aldosterone synthase (CYP11B2) gene polymorphisms, and gel Imaging Technology was used to determine the optimal annealing temperature.
方法：采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增，通过凝胶呈像技术判断CYP11 B2基因最佳退火温度。
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