In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity, a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.
为提高碱性磷酸酶(ALP)活性测定的灵敏度,对以4-甲基伞形酮磷酸酯(4-MUP)为底物的荧光法进行了研究。
Alkaline phosphatase (ALP) activity was evaluated by thymolphthalein release from thymolphthalein monophosphate and matrix mineralization by staining with alizarin red S.
用磷酸麝香草酚酞中释放麝香草酚酞方法检测碱性磷酸酶ALP活性用硫酸茜红素染色法检测基质矿化。
Alkaline phosphatase (ALP) activity of osteoblasts was determined by enzyme dynamical method at 2d, 4d and 6d .
采用酶动力法测成骨细胞在基质材料上培养2,4,6 天后的碱性磷酸酶(ALP)活性。
SEM, MTT assay and alkaline phosphatase (ALP) activity were carried out to observe the effect of AEXCB on cell growth, proliferation and functional expression.
通过扫描电镜、MTT测试法、考马斯亮蓝和碱性磷酸酶活性检测法,观察去抗原异种松质骨材料对细胞生长、增殖及功能表达的影响。
Alkaline phosphatase (ALP) activity was assayed as well as to assess gene expression by RT-PCR.
成骨诱导后检测碱性磷酸酶(ALP)活性,RT - P CR分析其成骨相关基因的表达情况。
OBJECTIVE:To investigate the effects of fibrin gel combined with bFGF on alkaline phosphatase activity(ALP)in fetal rat limb cells.
目的:观察纤维蛋白凝胶复合碱性成纤维细胞生长因子对大鼠胎肢细胞碱性磷酸酶活性的影响。
The serum estradiol(E2) was significantly decreased, the activity of serum alkaline phosphatase(ALP) and tartrate resistant acid phosphatase(TRAP) was markedly increased.
血清雌二醇水平显著降低,血清碱性磷酸酶和抗酒石酸酸性磷酸酶活性显著升高。
The serum estradiol(E2) was significantly decreased, the activity of serum alkaline phosphatase(ALP) and tartrate resistant acid phosphatase(TRAP) was markedly increased.
血清雌二醇水平显著降低,血清碱性磷酸酶和抗酒石酸酸性磷酸酶活性显著升高。
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