We have isolated a novel immunoglobulin type cell adhesion molecule, DSCAML1, from a fetal brain cDNA library.
在胎脑文库中获得的DSCAML1属于免疫球蛋白超家族类型的细胞粘附分子。
The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.
将文库菌落印迹至尼龙膜,分区培养提取质粒DNA ,建立基因池,并分别转染NIH/3T3细胞。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
根据计算机克隆的ZNF322基因序列设计引物,从人类胚胎心脏文库中克隆了ZNF322基因。
Some defense-relate genes were identified with cDNA library, they are possible related to resistances against coldness, drought etc in jujube.
测序得到了许多与抗逆性有关的蛋白,这可能与枣树本身抗寒,抗旱等抗逆性强有关。
RESULTS: human NS5ABP37 was screened and cloned from human liver cDNA library by yeast-two hybrid system 3. The murine NS5ABP37 was deduced by bioinformatics methods.
结果:通过酵母双杂交技术获得了人ns5abp37的编码基因。利用生物信息学技术确定了小鼠ns5abp37的基因序列。
Results A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed.
结果建立了从骨骼和关节构建RACE c DNA文库的方法,并用该方法成功地构建了人胎儿骨骼和关节RACE c DNA文库。
Objective To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.
目的分离晕船适应大鼠下丘脑差异表达基因片段,以探讨晕船适应机理。
Currently, several approaches have been developed for gene enrichment on a genome-wide scale, such as cDNA library, methylation filtration library, high Cot library and transposon tagging.
目前,在基因组范围内富集基因的方法有cD NA文库、甲基化过滤文库、高Cot值文库、转座子标签富集法等。
A library containing the genes that could be expressed as proteins would be referred to as a cDNA expression library, which could be screened for a protein that binds a particular antibody.
一个包含可表达为蛋白质的基因的文库称为cDNA表达文库,它可以用来筛选结合特定抗体的蛋白。
Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的:消减文库构建过程中,用P CR技术快速筛选重组阳性克隆。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
应用推荐