The Epidermal cell and fibroblasts culture in vitro were conducted by enzyme digestion. Epidermal cell primary culture was described by growth graph and MTT method was used in subculture cell growth.
酶消化法进行表皮细胞和成纤维细胞培养，绘制表皮细胞原代生长曲线，MTT法测定传代后表皮细胞的增殖。
Methods: The potassium currents of rat hippocampal neurons in primary culture were measured with patch clamp whole cell recording.
方法：膜片钳全细胞记录测量原代培养大鼠海马神经元膜的钾离子电流。
Objective To improve the primary cell culture methods of colorectal adenomas.
目的对大肠腺瘤细胞原代培养的方法作进一步的改进。
Primary cell culture was made from 23 malignant trophoblastic tumors and 19 normal early pregnant villi.
本文对23例恶性滋养细胞和19例正常早孕绒毛滋养细胞进行了原代培养。
We also established the cell line of this transgenic mice model through primary cell culture to make use this model more efficiently.
为了更为有效地利用该模型小鼠，我们通过原代培养建立了该模型的肿瘤细胞系。
Objective:To establish the quick and valid primary culture method of human placental villus in vitro and set up a human trophoblastic cell line with preliminary assessment.
目的：建立快速、有效的绒毛滋养细胞体外原代培养的方法以及建立人滋养细胞株和初步评价。
Methods: Primary embryonic rat cortical neurons culture, cytotoxicity assays and biochemistry detection, cell stain for the neuron morphology in microscopy and image analysis.
方法：原代胚胎鼠皮质神经元细胞培养，细胞毒性试验及生化指标测定，细胞染色，显微镜下观察形态学改变及影像分析。
Objective: To further investigate the mechanisms of hair cell generation or regeneration, the primary culture systems of cochlear sensory epithelial cell (CSEC) of rats were established.
目的：建立大鼠耳蜗感觉上皮细胞(CSEC)的原代培养体系，为研究毛细胞再生的分子机制提供大量来源一致的细胞。
Objective To observe the changes of limbal stem cell morphology? Antigen and proliferative ability after primary culture in vitro.
目的探讨人角膜缘干细胞体外培养后细胞形态学、抗原性和增殖能力的变化。
NECL2 distributed in cell body and protuberance of primary culture neuron. The rat adrenal gland tumor cell line PC12 can be induced into neuronal differentiation.
大鼠肾上腺嗜铬细胞瘤细胞PC12具有神经元样分化的潜能，在PC12细胞中转染并过量表达NECL2能够诱导细胞发生神经元样分化。
To establish the method on the primary culture of porcine intestinal epithelial cell, different digestion protocols and explant culture were used to identify the best method.
分别采用酶消化法和组织块培养法，建立了猪小肠黏膜上皮细胞原代培养的方法。
We further developed the primary cell culture if the tumor cells which would lead to better use of this genetic model.
同时还建立了原代培养的肿瘤细胞，提升了模型的利用效率。
Objective To study the inhibition of lung cancer cells of primary culture and A549 cell line on the proliferation of tumor-infiltrating lymphocytes (TIL) in vitro and its clinical implications.
目的研究肺癌原代细胞和细胞系a549对肿瘤浸润性淋巴细胞的抑制作用及其临床意义。
Methods: Using primary nerve cell culture, we observed the survival and growth of spinal cord neurons with phase-contract microscope.
方法：胚胎大鼠脊髓神经细胞原代培养，倒置相差显微镜下进行细胞记数和显微测量，观察神经元存活和生长分化的状况。
METHODS:Methods of in vitro cell culture model of human primary PDLC, tetrazolium bromide reduction (MTT) assay and direct contact were used.
方法：体外细胞培养，MTT （噻唑蓝）比色法，材料直接接触法。
Objective: to establish a human hepatocellular carcinoma cell line EHH1 by primary culture and to investigate its biological characteristics.
目的：采用原代培养的方法建立一株人肝癌细胞系ehh1，并对其生物学特性进行分析。
The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.
移植后肿瘤细胞在形态、生长、分泌CA12 5功能方面均与原细胞株保持一致。
The morphology, growth pattern and CA125 secretion of primary culture of transplanted cells remained as same as those of ovarian carcinoma cell line SKOV3.
移植后肿瘤细胞在形态、生长、分泌CA12 5功能方面均与原细胞株保持一致。
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