Methods: MTT assay, optical microscopy, electron microscopy, DNA agarose gel electrophoresis and in situ DNA nick end labeling were used to detect the apoptosis in BIU87/ADM and BIU87 cells.
方法:用噻唑蓝(MTT)法、光镜及电镜、DNA凝胶电泳和原位DNA末端转移酶标记法,分别对耐药细胞株BIU-87/ADM及敏感细胞株BIU-87细胞进行了阿霉素诱导的细胞凋亡研究。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
方法:四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率,应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
Results:A single band was obtained on agarose gel electrophoresis only from PCR products that contained genomic DNA of P. aeruginosa.
结果:PCR产物经琼脂糖凝胶电泳分析,仅在包含绿脓杆菌基因组DNA的样品中得到单一条带。
Methods: DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis.
方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定dna的损伤程度。
Restricted DNA products were then separated by useng 3% agarose gel electrophoresis and visuslized by UV light.
酶切产物经3%琼脂糖凝胶电泳分离后,紫外灯下检测酶切结果。
Agarose gel electrophoresis of the DNA extrate from cells treated with ActD shows typical DNA ladder.
DNA琼脂凝胶电泳呈典型的凋亡细胞DNA梯形条带;
Then DNA ladder zone of apoptosis cells was analyzed with the method of agarose gel electrophoresis.
用琼脂糖凝胶电泳法分析凋亡细胞的DNA梯带。
Soil microbial total DNA could not be obtained through DNA gel extraction kit after purification with 1% Sepharose containing 2% of PVP (polyvinylpyrrolidone) agarose gel electrophoresis.
含2 %PVP的1 %琼脂糖凝胶电泳纯化,用DNA凝胶回收试剂盒回收后没有得到纯化后的土壤微生物总DNA。
The characteristic DNA ladder was found by the agarose gel electrophoresis for the nucleus DNA of transfected cells.
细胞核DNA琼脂糖凝胶电泳显示凋亡细胞特有的梯状条带。
The potential of adsorbing DNA on nanoparticles was analyzed by agarose gel electrophoresis and spectrophotometer.
经琼脂糖凝胶电泳分析载体与DNA结合力,并通过紫外分光光度计检测其载药量和包埋率;
Methods Agarose gel electrophoresis of plasmid DNA, adherence test, and extract of plasmid were used. Electron microscope photos were prepared and observed.
方法:应用琼脂糖凝胶电泳试验、电镜照片制备及观察、粘附试验及质粒消除试验。
The results of agarose gel electrophoresis and DNA sequence analysis indicated that the PCR method had high specificity and sensitivity.
结果经琼脂糖凝胶电泳和DNA序列测定证实,建立的PCR检测方法具有极高的灵敏度和较好的特异性。
Methods Flow cytometry were used to evaluate HepG2 cells apoptosis. agarose gel electrophoresis was used to detected DNA fragmentation;
方法流式细胞术检测细胞凋亡;琼脂糖凝胶电泳检测断裂DNA;
Method:The DNA gradent bands were detected by agarose gel electrophoresis.
方法:用琼脂糖凝胶电泳法观察DNA的梯状条带。
The interaction with DNA and bovine serum albumin (BSA) were studied by fluorescence spectra and agarose gel electrophoresis.
运用荧光光谱法、琼脂糖凝胶电泳法分别研究了配合物与DNA和BSA的作用。
Methods:By checking the morphology of apoptotic cells, agarose gel electrophoresis of DNA fragmentation and quantitation of apoptotic cells by means of flow cytometric analysis.
方法:应用细胞形态学检查,DNA凝胶电泳及流式细胞仪分析检测。
DNA fragmentation detected by agarose gel electrophoresis;
琼脂糖凝胶电泳检测断裂DNA ;
DNA fragmentation detected by agarose gel electrophoresis;
琼脂糖凝胶电泳检测断裂DNA ;
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